Open Access
Research (Published online: 28-10-2022)
13. Sangyod rice bran extract enhances Lacticaseibacillus paracasei growth during the exponential phase and antibacterial activity of L. paracasei supernatant against zoonotic and foodborne pathogens
Krittika Kabploy, Phirabhat Saengsawang, Chonticha Romyasamit, Suthinee Sangkanu, Warangkana Kitpipit, Thotsapol Thomrongsuwannakij, Tuempong Wongtawan, Mareena Daus, Maria de Lourdes Pereira, and Watcharapong Mitsuwan
Veterinary World, 15(10): 2466-2474

Krittika Kabploy: School of Agricultural Technology and Food Industry, Walailak University, Nakhon Si Thammarat 80160, Thailand; Center of Excellence in Innovation of Essential Oil and Bioactive Compounds, Walailak University, Nakhon Si Thammarat, 80160, Thailand.
Phirabhat Saengsawang: Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat, 80160, Thailand; One Health Research Center, Walailak University, Nakhon Si Thammarat, 80160, Thailand.
Chonticha Romyasamit: Center of Excellence in Innovation of Essential Oil and Bioactive Compounds, Walailak University, Nakhon Si Thammarat, 80160, Thailand; School of Allied Health Sciences and World Union for Herbal Drug Discovery (WUHeDD), Walailak University, Nakhon Si Thammarat, Thailand.
Suthinee Sangkanu: School of Allied Health Sciences and World Union for Herbal Drug Discovery (WUHeDD), Walailak University, Nakhon Si Thammarat, Thailand.
Warangkana Kitpipit: Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat, 80160, Thailand; One Health Research Center, Walailak University, Nakhon Si Thammarat, 80160, Thailand; Food Technology and Innovation Center of Excellence, Walailak University, Nakhon Si Thammarat, 80160, Thailand.
Thotsapol Thomrongsuwannakij: Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat, 80160, Thailand; One Health Research Center, Walailak University, Nakhon Si Thammarat, 80160, Thailand.
Tuempong Wongtawan: Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat, 80160, Thailand; One Health Research Center, Walailak University, Nakhon Si Thammarat, 80160, Thailand.
Mareena Daus: Division of Physical Science and Natural Products Center of Excellent, Faculty of Science, Prince of Songkla University, Songkhla, 80160, Thailand.
Maria de Lourdes Pereira: CICECO-Aveiro Institute of Materials and Department of Medical Sciences, University of Aveiro, Aveiro, 3810-193, Portugal.
Watcharapong Mitsuwan: Center of Excellence in Innovation of Essential Oil and Bioactive Compounds, Walailak University, Nakhon Si Thammarat, 80160, Thailand; Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat, 80160, Thailand; One Health Research Center, Walailak University, Nakhon Si Thammarat, 80160, Thailand.

doi: www.doi.org/10.14202/vetworld.2022.2466-2474

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Article history: Received: 03-06-2022, Accepted: 13-09-2022, Published online: 28-10-2022

Corresponding author: Watcharapong Mitsuwan

E-mail: watcharapong.mi@wu.ac.th

Citation: Kabploy K, Saengsawang P, Romyasamit C, Sangkanu S, Kitpipit W, Thomrongsuwannakij T, Wongtawan T, Daus M, Pereira ML, and Mitsuwan W (2022) Sangyod rice bran extract enhances Lacticaseibacillus paracasei growth during the exponential phase and antibacterial activity of L. paracasei supernatant against zoonotic and foodborne pathogens, Veterinary World, 15(10): 2466–2474.
Abstract

Background and Aim: Prebiotics are a group of nutrients or compounds that are degraded by the gut microbiota, including Lacticaseibacillus paracasei. The probiotic plays an important role in adhesion to the gut and is able to produce antimicrobial substances to inhibit pathogens. This study aimed to investigate the effects of Sangyod rice bran extract on the growth promotion of L. paracasei. Furthermore, antibacterial activity of the extract and L. paracasei supernatants cultured in De Man, Rogosa and Sharpe (MRS) medium plus the extract against zoonotic and foodborne pathogens was investigated.

Materials and Methods: Antibacterial activity of the crude extract and the oil from Sangyod rice bran against the pathogens, including Bacillus cereus, Staphylococcus aureus, Escherichia coli, Avian pathogenic E. coli, and Pseudomonas aeruginosa was investigated using broth microdilution assay. The effects of the crude extract and the oil on the growth and adhesion of L. paracasei were further determined. The antibacterial activity of L. paracasei supernatant cultured in the medium supplemented with the extract and the oil against the pathogens was determined by agar well diffusion assay, followed by the broth microdilution assay. Finally, the chemical constituents and antioxidant activity of the crude extract and the oil from Sangyod rice bran were investigated.

Results: The crude extract and the oil from Sangyod rice bran enhanced L. paracasei growth during the exponential phase. Furthermore, the crude extract at 0.25 mg/mL significantly enhanced the adhesion of L. paracasei to the surface compared with the control. Both minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values of the crude extract against B. cereus and S. aureus were 0.5 and 1.0 mg/mL, respectively. All pathogens were sensitive to the supernatant of L. paracasei with similar MIC and MBC ranging from 12.5% v/v to 50% v/v. However, the MIC and MBC values of L. paracasei supernatant grown in MRS medium plus the crude extract and oil were not significantly different compared to the supernatant obtained from MRS alone. The crude extract had free radical scavenging activities with IC50 values at 0.61 mg/mL.

Conclusion: The results suggested the potential benefits of the crude extract from Sangyod rice bran for inducing the growth and the adhesion of L. paracasei and inhibiting zoonotic and foodborne pathogens.

Keywords: Avian pathogenic Escherichia coli, Bacillus cereus, Lacticaseibacillus paracasei, Sangyod rice bran extract, Staphylococcus aureus.