Effect of leptin on the growth and expression of STAT3 in yak mammary epithelial cells

Research (Published online: 07-09-2022)

4. Effect of leptin on the growth and expression of STAT3 in yak mammary epithelial cells

Baoxia Dong, Sidra Mehran, Yuying Yang, Haixia Jing, Lin Liang, Xiaoyu Guo, and Qinwen Zhang

Veterinary World, 15(9): 2141-2150

Baoxia Dong: Department of Animal Medicine, College of Agriculture and Animal Husbandry, Qinghai University, Xining, China.

Sidra Mehran: Department of Animal Medicine, College of Agriculture and Animal Husbandry, Qinghai University, Xining, China.

Yuying Yang: Department of Animal Medicine, College of Agriculture and Animal Husbandry, Qinghai University, Xining, China.

Haixia Jing: Department of Animal Medicine, College of Agriculture and Animal Husbandry, Qinghai University, Xining, China.

Lin Liang: Department of Biotechnology, Kunlun College, Qinghai University, Xining, China.

Xiaoyu Guo: Department of Animal Medicine, College of Agriculture and Animal Husbandry, Qinghai University, Xining, China.

Qinwen Zhang: Department of Animal Medicine, College of Agriculture and Animal Husbandry, Qinghai University, Xining, China.

doi: www.doi.org/10.14202/vetworld.2022.2141-2150

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Article history: Received: 18-06-2022, Accepted: 08-08-2022, Published online: 07-09-2022

Corresponding author: Haixia Jing

E-mail: jinghaixia1@163.com

Citation: Dong B, Mehran S, Yang Y, Jing H, Liang L, Guo X, and Zhang Q (2022) Effect of leptin on the growth and expression of STAT3 in yak mammary epithelial cells, Veterinary World, 15(9): 2141–2150.

Abstract

Background and Aim: Leptin (LEP) is an autocrine and paracrine factor produced by the fat pad and acinar epithelial cells of the breast. This study aimed to investigate the effects of LEP on yak mammary epithelial cells (YMECs) and the expression of STAT3. In addition, we evaluated the possible effects of prolactin (PRL) on the function of LEP.

Materials and Methods: The YMECs were treated with 0, 50, 100, 200, 400, and 800 ng/mL LEP for 48 h in the absence of PRL and the presence of 500 ng/mL PRL. The growth activity of YMECs was measured using the cell counting kit-8 assay. The changes in the lactation signaling pathway-related factor STAT3 were detected at the mRNA, protein, and protein phosphorylation levels using the reverse transcriptase-quantitative polymerase chain reaction and Western blotting. To explore whether LEP affects the activation of STAT3 through JAK2/JAK3 in YMECs, the JAK2/3 signaling pathway inhibitor AG490 was used at a fixed concentration of LEP.

Results: Each concentration of LEP significantly promoted the expression of STAT3 mRNA (p < 0.05) in YMECs in the presence of PRL. In the absence of PRL, all concentrations of LEP were found to inhibit the expression of the STAT3 protein (p < 0.05). The expression of the STAT3 protein in YMECs was found to first increase followed by a decrease with an increase in the concentration of LEP. In addition, the phosphorylation level of STAT3 increased in all groups, except the 100 ng/mL concentration group. The STAT3 phosphorylation trend and protein expression were different, such that the level of protein phosphorylation was higher than that of the STAT3 protein (p < 0.05). The addition of AG490 reduced the expression of the STAT3 mRNA, STAT3 protein, and STAT3 phosphorylation in the LEP and LEP + PRL groups.

Conclusion: Altogether, the results indicated that different concentrations of LEP exerted varying effects on the growth of YMECs and the expression of STAT3, and the activity of STAT3 was primarily activated by JAK2. The addition of LEP can effectively inhibit the downregulation of the JAK2/STAT3 signal pathway by AG490, mitigate its inhibitory effect on the proliferation of YMECs, and reduce apoptosis. We believe that these findings will provide a theoretical and experimental basis for future research in this field.

Keywords: JAK2, leptin, mammary epithelial cells, STAT3, yak.