Cuticular surface damage of Ascaridia galli adult worms treated with Veitchia merrillii betel nuts extract in vitro

Vet World Vol.10 July-2017 Article-3

Research Article

Veterinary World, 10(7): 732-737

https://doi.org/10.14202/vetworld.2017.732-737

Ummu Balqis¹, Muhammad Hambal², Rinidar³, Farida Athaillah², Ismail⁴, Azhar⁵, Henni Vanda³, and Darmawi^6,7

1. Laboratory of Pathology, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Aceh, Indonesia.
2. Laboratory of Parasitology, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Aceh, Indonesia.
3. Laboratory of Pharmacology, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Aceh, Indonesia.
4. Laboratory of Veterinary Public Health, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Aceh, Indonesia.
5. Laboratory of Physiology, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Aceh, Indonesia.
6. Laboratory of Research, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Aceh, Indonesia.
7. Laboratory of Microbiology, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Aceh, Indonesia.

Background and Aim: The objective of this research was to in vitro evaluate the cuticular surface damage of Ascaridia galli adult worms treated with ethanolic extract of betel nuts Veitchia merrillii.

Materials and Methods: Phytochemical screening was done using FeCl3, Wagner and Dragendorff reagents, NaOH, MgHCl, and Liebermann-Burchard reaction test. Amount of 16 worms were segregated into four groups with three replicates. Four worms of each group submerged into phosphate buffered saline, 25 mg/ml, and 75 mg/ml crude ethanolic extract of V. merrillii, and 15 mg/ml albendazole. The effect of these extract was observed 40 h after incubation as soon as worms death. The worms were sectioned transversally and were explored for any cuticular histopathological changes in their body surface under microscope.

Results: We found that the ethanolic extract of V. merrillii betel nuts contains tannins, alkaloids, flavonoids, triterpenoids, and saponins. The ethanolic extract of betel nuts V. merrillii induces surface alterations caused cuticular damage of A. galli adult worms.

Conclusion: We concluded that ethanolic extract of betel nuts V. merrillii possess anthelmintic activity caused cuticular damage of A. galli adult worms. Keywords: anthelmintics, Ascaridia galli, cuticle, Veitchia merrillii.

Keywords: anthelmintics, Ascaridia galli, cuticle, Veitchia merrillii.

How to cite this article: Balqis U, Hambal M, Rinidar, Athaillah F, Ismail, Azhar, Vanda H, Darmawi (2017) Cuticular surface damage of Ascaridia galli adult worms treated with Veitchia merrillii betel nuts extract in vitro, Veterinary World, 10(7): 732-737.

Received: 14-12-2016 Accepted: 17-05-2017 Published online: 03-07-2017

Corresponding author: Darmawi E-mail: darmawi@unsyiah.ac.id

DOI: 10.14202/vetworld.2017.732-737

Copyright: Balqis, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.