Evaluation of crude larval protein and recombinant somatic protein 26/23 (rHcp26/23) immunization against Haemonchus contortus in sheep

Vet World Vol.10 July-2017 Article-8

Research Article

Veterinary World, 10(7): 758-763

https://doi.org/10.14202/vetworld.2017.758-763

Omnia M. Kandil¹, Khaled A. Abdelrahman¹, Hatem A. Shalaby¹, Seham H. M. Hendawy¹, Nadia M. T. Abu El Ezz¹, Somia A. Nassar¹, and James E. Miller²

1. Department of Parasitology and Animal Diseases, National Research Centre, Dokki, P.O. Box 12622, Giza, Egypt.
2. Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.

Background and Aim: The aim of this study was to evaluate the potential possibility of crude larval and recombinant (rHcp26/23) antigens of Haemonchus contortus for immunization to control sheep hemonchosis.

Materials and Methods: A total of 21 lambs were divided into five groups. Lambs were immunized with larval and recombinant (rHcp26/23) proteins at day 0 and day 14 and after that challenged with 5000 infective larvae of H. contortus on day 42. An unvaccinated positive control group was challenged with L3 in the meantime. An unvaccinated negative control group was not challenged.

Results: Fecal egg count reduction taking after challenge for rHcp26/23 and larval antigens was 92.2% and 38.2%, respectively, compared with the positive control group. Vaccine incited protection in rHcp26/23 and larval immunization was reflected in significant (p<0.05) decreases in worm burden; 59.9% and 40.1%, respectively.

Conclusion: Recombinant rHcp26/23 vaccine induced a partial immune response and had immune-protective effect against sheep hemonchosis. Keywords: antigen, Haemonchus contortus, immunization, larval, rHcp26/23, sheep.

Keywords: antigen, Haemonchus contortus, immunization, larval, rHcp26/23, sheep.

How to cite this article: Kandil OM, Abdelrahman KA, Shalaby HA, Hendawy SHM, Abu El Ezz NMT, Nassar SA, Miller JE (2017) Evaluation of crude larval protein and recombinant somatic protein 26/23 (rHcp26/23) immunization against Haemonchus contortus in sheep, Veterinary World, 10(7): 758-763.

Received: 10-01-2017 Accepted: 24-05-2017 Published online: 08-07-2017

Corresponding author: Omnia M. Kandil E-mail: kandil_om@yahoo.com

DOI: 10.14202/vetworld.2017.758-763

Copyright: Kandil, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.