Vet World Vol.10 June-2017 Article-14
Research Article
Veterinary World, 10(6): 655-661
https://doi.org/10.14202/vetworld.2017.655-661
Hemogram responses in goats toward challenged with Corynebacterium pseudotuberculosis and its immunogen mycolic acids
2. Department of Veterinary Medicine, Faculty of Agriculture and Veterinary Medicine, Thamar University, Dhamar, Yemen.
3. Department of Veterinary Clinical Studies, Research Center for Ruminant Disease, Universiti Putra Malaysia 43400 Serdang Selangor, Malaysia.
4. Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
5. Department of Veterinary Public Health and Preventive Medicine, University of Maiduguri, Nigeria.
6. Department of Animal Reproduction, Faculty of Verterinary and Animal Sciences, University of Agriculture, Water and Marine Sciences, Uthal, Baluchistan, Pakistan.
7. Department of Internal and Preventive Medicine, Faculty of Veterinary Medicine, University of Basra, Basra, Iraq.
Background and Aim: This study was conducted to analyze the changes in blood profile of goats inoculated with Corynebacterium pseudotuberculosis and its immunogen mycolic acid (MA) extract.
Materials and Methods: A total of 12 clinically healthy crossbred Boer female goats were divided into three groups; A, B and C (4 goats each per group). Group A was inoculated with 2 ml sterile phosphate buffered saline via intradermal route as the negative control group whilst Group B was inoculated with 2 ml of MA extract (1 g/ml) intradermally and Group C was then inoculated with 2 ml (1x109) colony forming unit of active C. pseudotuberculosis intradermally. Blood sample was collected aseptically from the jugular vein periodically for complete blood count (CBC) analysis throughout the experimental period (3 months).
Results: A significant decrease (p<0.05) was observed in red blood cells, hemoglobin (Hb), packed cell volume, mean corpuscular volume and mean corpuscular Hb concentration in Groups B and C as compared to the control while WBCs, neutrophil, lymphocyte and basophil showed a significant increase (p<0.05) as compared to the control.
Conclusion: The inoculation of C. pseudotuberculosis and MA resulted in a significant change in the CBC, thereby, indicating that MA has a role in caseous lymphadenitis pathogenesis. Keywords: complete blood count, Corynebacterium pseudotuberculosis, goat, hematology, mycolic acid.
Keywords: complete blood count, Corynebacterium pseudotuberculosis, goat, hematology, mycolic acid.
How to cite this article: Odhah MN, Abdullah FFJ, Haron AW, Mohd. Lila MA, Zamri-Saad M, Khuder Z, Hambali IU, Umar M, Saleh WM (2017) Hemogram responses in goats toward challenged with Corynebacterium pseudotuberculosis and its immunogen mycolic acids, Veterinary World, 10(6): 655-661.
Received: 25-11-2016 Accepted: 21-03-2017 Published online: 17-06-2017
Corresponding author: Faez Firdaus Jesse Abdullah E-mail: jesse@upm.edu.my
DOI: 10.14202/vetworld.2017.655-661
Copyright: Odhah, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.