Immunoglobulin subclass in experimental murine Toxocara cati infection

Vet World Vol.10 November-2017 Article-9

Research Article

Veterinary World, 10(11): 1333-1336

https://doi.org/10.14202/vetworld.2017.1333-1336

Kusnoto¹, Setiawan Koesdarto¹, Sri Mumpuni¹, and R. Heru Prasetyo²

1. Department of Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia.
2. Department of Parasitology, Faculty of Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia.

Background and Aim: The aim of this study was to detect specific immunoglobulin (Ig) that could be used to determine monoclonal antibody in conjugate-making an effort for the indirect enzyme-linked immunosorbent assay (ELISA) diagnostic kit of toxocariasis in human.

Materials and Methods: The study was conducted to assess the Ig profile, based on ELISA-isotyping, in mice infected with second stage larvae eggs of Toxocara cati. The optical density values of anti-T. cati mice serum IgG subclasses were analyzed by applying ANOVA factorial.

Results: The specific IgG subclass in mice infected with T. cati mice was found to be IgG2β.

Conclusion: Subclass of IgG, especially IgG2β, can provide leads about the use of the monoclonal antibody in conjugate making an effort for the indirect ELISA diagnostic kit. Keywords: diagnostic kit, enzyme-linked immunosorbent assay, human, immunoglobulin G subclass, monoclonal antibody, toxocariasis, Toxocara cati.

Keywords: diagnostic kit, enzyme-linked immunosorbent assay, human, immunoglobulin G subclass, monoclonal antibody, toxocariasis, Toxocara cati.

How to cite this article: Kusnoto, Koesdarto S, Mumpuni S, Prasetyo RH (2017) Immunoglobulin subclass in experimental murine Toxocara cati infection, Veterinary World, 10(11): 1333-1336.

Received: 20-06-2017 Accepted: 23-10-2017 Published online: 13-11-2017

Corresponding author: R. Heru Prasetyo E-mail: rheru_prasetyo@yahoo.co.id

DOI: 10.14202/vetworld.2017.1333-1336

Copyright: Kusnoto, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.