Pathology and immunohistochemistry study of Newcastle disease field case in chicken in Indonesia

Vet World Vol.10 September-2017 Article-10

Research Article

Veterinary World, 10(9): 1066-1071

https://doi.org/10.14202/vetworld.2017.1066-1071

Etriwati¹, Dewi Ratih², Ekowati Handharyani², and Surachmi Setiyaningsih³

1. Laboratory of Pathology, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Indonesia.
2. Department of Clinic Reproduction and Pathology, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia.
3. Department of Animal Disease and Public Health, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia.

Background and Aim: The aim of the study was to examine pathology and the distribution pattern of Newcastle disease virus (NDV) in internal organs of chickens from a field case using immunohistochemical staining.

Materials and Methods: 10 groups of broiler, layer, and domestic chicken were collected from necropsy room Division of Pathology, Bogor Agricultural University. These chickens were originated from West Java and collected based on pathologist diagnosis as suspect of Newcastle disease (ND). They were subsequently confirmed positive of ND with real-time-reverse transcription polymerase chain reaction assay. The respiratory, circulatory, digestive, lymphoreticular and central nervous systems were collected for histopathology examination.

Results: The gross pathology and histopathology changes were tracheitis, pneumonia, pericarditis, myocarditis, catarrhal proventriculitis, catarrhal enteritis, typhlitis, perihepatitis, pancreatitis, nephritis interstitial, splenitis, atrophy of Bursa Fabricius, and encephalitis.

Conclusion: The distribution pattern of NDV in internal organs of chickens from a field case in this study is similar with a previous reported pattern in systemic cases of the internal chicken organs. High intensity of immunohistochemistry stain result was detected in trachea, lung, proventriculus, duodenum, cecal tonsil, kidney, and brain. Keywords: broiler, domestic chicken, immunohistochemistry, layer, Newcastle disease.

Keywords: broiler, domestic chicken, immunohistochemistry, layer, Newcastle disease.

How to cite this article: Etriwati, Ratih D, Handharyani E, Setiyaningsih S (2017) Pathology and immunohistochemistry study of Newcastle disease field case in chicken in Indonesia, Veterinary World, 10(9): 1066-1071.

Received: 21-04-2017 Accepted: 18-08-2017 Published online: 13-09-2017

Corresponding author: Etriwati E-mail: etriwati.2102@unsyiah.ac.id

DOI: 10.14202/vetworld.2017.1066-1071

Copyright: Etriwati, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.