Amino acid sequence based on Cytochrome b gene in Kejobong goat and its genetic relationships among several local goats in Asia

Vet World Vol.11 August-2018 Article-27

Research Article

Veterinary World, 11(8): 1196-1202

https://doi.org/10.14202/vetworld.2018.1196-1202

Dela Ayu Lestari, Endang Purbowati, Sutopo Sutopo, and Edy Kurnianto

Department of Animal Science, Faculty of Animal and Agricultural Sciences, Diponegoro University, Tembalang Campus, Semarang 50275, Central Java, Indonesia.

Background and Aim: This study aimed to analyze the amino acid sequence of Cytochrome b (Cyt b) gene in Kejobong goat and its genetic relationships with local goats located in Asia.

Materials and Methods: A total of 28 heads of Kejobong goat were purposively sampled. The deoxyribonucleic acid (DNA) was extracted from blood using gSYNC DNA mini kit (Geneaid Biotech Ltd.). Cyt b gene was amplified using polymerase chain reaction (PCR) method with CytbCapF and CytbCapR primers. The amplified PCR products were sequenced for further analysis.

Results: There were a total 377 amino acid sequences translated from 1140 base pair (bp) of Cyt b gene, 99.20% of it were monomorphic, amino acid alterations were found at site 16th, 121st, and 231st, and Kejobong goat was in the same cluster with Southeast Asian local goats.

Conclusion: Most of the amino acid sequence on Cyt b gene in Kejobong goat is monomorphic (99.20%), only a few nucleotide mutations were found that causing amino acid alteration in three sites (0.80%). Kejobong goat has a close genetic relationship to several local goats in Southeast Asian. Keywords: amino acid sequence, cytochrome b, Kejobong goat.

Keywords: amino acid sequence, cytochrome b, Kejobong goat.

How to cite this article: Lestari DA, Purbowati E, Sutopo S, Kurnianto E (2018) Amino acid sequence based on Cytochrome b gene in Kejobong goat and its genetic relationships among several local goats in Asia, Veterinary World, 11(8): 1196-1202.

Received: 05-04-2018 Accepted: 13-07-2018 Published online: 30-08-2018

Corresponding author: Edy Kurnianto E-mail: kurniantoedy17@gmail.com

DOI: 10.14202/vetworld.2018.1196-1202

Copyright: Lestari, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.