Vet World Vol.11 August-2018 Article-27
Research Article
Veterinary World, 11(8): 1196-1202
https://doi.org/10.14202/vetworld.2018.1196-1202
Amino acid sequence based on Cytochrome b gene in Kejobong goat and its genetic relationships among several local goats in Asia
Background and Aim: This study aimed to analyze the amino acid sequence of Cytochrome b (Cyt b) gene in Kejobong goat and its genetic relationships with local goats located in Asia.
Materials and Methods: A total of 28 heads of Kejobong goat were purposively sampled. The deoxyribonucleic acid (DNA) was extracted from blood using gSYNC DNA mini kit (Geneaid Biotech Ltd.). Cyt b gene was amplified using polymerase chain reaction (PCR) method with CytbCapF and CytbCapR primers. The amplified PCR products were sequenced for further analysis.
Results: There were a total 377 amino acid sequences translated from 1140 base pair (bp) of Cyt b gene, 99.20% of it were monomorphic, amino acid alterations were found at site 16th, 121st, and 231st, and Kejobong goat was in the same cluster with Southeast Asian local goats.
Conclusion: Most of the amino acid sequence on Cyt b gene in Kejobong goat is monomorphic (99.20%), only a few nucleotide mutations were found that causing amino acid alteration in three sites (0.80%). Kejobong goat has a close genetic relationship to several local goats in Southeast Asian. Keywords: amino acid sequence, cytochrome b, Kejobong goat.
Keywords: amino acid sequence, cytochrome b, Kejobong goat.
How to cite this article: Lestari DA, Purbowati E, Sutopo S, Kurnianto E (2018) Amino acid sequence based on Cytochrome b gene in Kejobong goat and its genetic relationships among several local goats in Asia, Veterinary World, 11(8): 1196-1202.
Received: 05-04-2018 Accepted: 13-07-2018 Published online: 30-08-2018
Corresponding author: Edy Kurnianto E-mail: kurniantoedy17@gmail.com
DOI: 10.14202/vetworld.2018.1196-1202
Copyright: Lestari, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.