Research Article | 30 Aug 2018

Amino acid sequence based on Cytochrome b gene in Kejobong goat and its genetic relationships among several local goats in Asia

Dela Ayu Lestari, Endang Purbowati, Sutopo Sutopo, and Edy KurniantoShow more
VETERINARY WORLD | pg no. 1196-1202 | Vol. 11, Issue 8 | DOI: 10.14202/vetworld.2018.1196-1202
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Abstract

          
Background and Aim: This study aimed to analyze the amino acid sequence of Cytochrome b (Cyt b) gene in Kejobong goat and its genetic relationships with local goats located in Asia.
Materials and Methods: A total of 28 heads of Kejobong goat were purposively sampled. The deoxyribonucleic acid (DNA) was extracted from blood using gSYNC DNA mini kit (Geneaid Biotech Ltd.). Cyt b gene was amplified using polymerase chain reaction (PCR) method with CytbCapF and CytbCapR primers. The amplified PCR products were sequenced for further analysis.
Results: There were a total 377 amino acid sequences translated from 1140 base pair (bp) of Cyt b gene, 99.20% of it were monomorphic, amino acid alterations were found at site 16th, 121st, and 231st, and Kejobong goat was in the same cluster with Southeast Asian local goats.
Conclusion: Most of the amino acid sequence on Cyt b gene in Kejobong goat is monomorphic (99.20%), only a few nucleotide mutations were found that causing amino acid alteration in three sites (0.80%). Kejobong goat has a close genetic relationship to several local goats in Southeast Asian. Keywords: amino acid sequence, cytochrome b, Kejobong goat.
          
Keywords: amino acid sequence, cytochrome b, Kejobong goat.