Vet World Vol.11 August-2018 Article-8
Research Article
Veterinary World, 11(8): 1064-1073
https://doi.org/10.14202/vetworld.2018.1064-1073
Effectiveness of poultry litter amendments on bacterial survival and Eimeria oocyst sporulation
2. Department of Parasitology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt.
Background and Aim: Broilers' optimum performance in response to their genetic potential depends on litter environment which is ideal for bacterial survival and coccidian oocyst sporulation. An in vitro evaluation was conducted for the effectiveness of superphosphate, meta-bisulfide, and charcoal litter amendments in minimizing Escherichia coli O157:H7 and Salmonella Typhimurium survival, Eimeria oocyst count, and sporulation.
Materials and Methods: Three groups of 16 litter trays were prepared and inoculated with E. coli O157:H7, S. Typhimurium, and Eimeria non-sporulated oocyst. A set of four trays in each group was designed for each one of the chemical amendments. A total of 720 litter samples were collected and examined for bacterial counts, Eimeria oocyst count, and sporulation during the experimental period (35 days).
Results: Litter moisture and pH revealed a highly significant (p<0.001) reduction in all treated litter trays compared to control. Total bacterial count (TBC), total Enterobacteriaceae count, and S. Typhimurium count showed a highly significant (p<0.001) reduction in meta-bisulfide-treated trays compared to other amendments and positive control. Meanwhile, Eimeria oocyst count and sporulation revealed a highly significant (p<0.001) reduction in superphosphate, meta-bisulfide, and charcoal-treated trays, respectively. Temperature revealed a highly significant (p<0.001) weak positive correlation with pH of all inoculated trays, a highly significant (p<0.001) weak negative correlation with moisture percentage of E. coli O157:H7 and S. Typhimurium inoculated trays, and a highly significant (p<0.001) weak negative correlation with TBC. Meanwhile, relative humidity revealed significant (p≤0.005) weak positive correlation with moisture percentage of E. coli O157:H7 inoculated trays.
Conclusion: The study concluded that regular usage with periodical reapplication of litter amendments as meta-bisulfide or superphosphate in poultry farms is one of the indispensable managemental and preventive measures for minimizing bacterial survival and inhibiting Eimeria oocyst maturation and sporulation. Keywords: charcoal, Eimeria, Escherichia coli, litter, meta-bisulfide, Salmonella, superphosphate.
Keywords: charcoal, Eimeria, Escherichia coli, litter, meta-bisulfide, Salmonella, superphosphate.
How to cite this article: Soliman ES, Sallam NH, Abouelhassan EM (2018) Effectiveness of poultry litter amendments on bacterial survival and Eimeria oocyst sporulation, Veterinary World, 11(8): 1064-1073.
Received: 14-03-2018 Accepted: 25-06-2018 Published online: 06-08-2018
Corresponding author: Essam S. Soliman E-mail: soliman.essam@vet.suez.edu.eg
DOI: 10.14202/vetworld.2018.1064-1073
Copyright: Soliman, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.