Phenotypic and genotypic drug resistance profile of Salmonella serovars isolated from poultry farm and processing units located in and around Mumbai city, India

Vet World Vol.11 December-2018 Article-6

Research Article

Veterinary World, 11(12): 1682-1688

https://doi.org/10.14202/vetworld.2018.1682-1688

R. N. Waghamare¹, A. M. Paturkar¹, V. M. Vaidya¹, R. J. Zende¹, Z. N. Dubal², A. Dwivedi², and R. V. Gaikwad¹

1. Department of Veterinary Public Health, Bombay Veterinary College, Parel, Mumbai, Maharashtra, India.
2. Department of Veterinary Public Health, ICAR - Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India.

Background and Aim: The extensive use of antimicrobials in poultry has led to an increase in bacterial multidrug resistance, and the emergence of multidrug-resistant nontyphoidal Salmonella is a global problem. This study was performed to detect antibiotic-resistant Salmonella serovars in poultry farming and processing environment.

Materials and Methods: A total of 956 various samples, comprising 432 farm origin, 324 poultry processing stage wise and environmental, and 154 product processing stages and environmental samples, were collected from poultry farms and processing units located in and around Mumbai city. Of a total of 71 recovered isolates, 42 randomly selected Salmonella isolates were subjected for antibiotic susceptibility testing by disk diffusion method and serotyping. A total of 31 serotypically confirmed isolates were characterized for the presence of tetA, tetB, blaTEM, and CTX-M gene.

Results: Higher resistance was recorded against Doxycycline (100%), followed by Oxytetracycline (97.62%), Neomycin (88.10%), Erythromycin (83.33%), Tetracycline (78.57%), and Ceftizoxime (35.71%). Resistance from 0.00 to 26.19 percent was found to antimicrobials, namely Norfloxacin (26.19%), Ampicillin (21.43%), Azithromycin (21.43%), Ciprofloxacin (19.05%), Colistin (4.76%), Streptomycin (16.67%), Cefotaxime (14.19%), Enrofloxacin (14.29%), Amoxyclav (14.29%), Gentamicin (7.14%), Chloramphenicol (4.76%), Amikacin (4.76%), and Ceftazidime (0.0%). Results demonstrate that the Salmonella Virchow dominated and all serotypes were found to carry Tetracycline resistance gene tetA, 5 isolates were found to be positive for blaTEM, whereas none of the isolates were carrying tetB and CTX-M gene.

Conclusion: This study revealed that there is a significant rise of Tetracycline resistance with the presence of tetA gene in Salmonella spp. which indicates selective pressure for adopting resistance against tetracycline group of antibiotics. Keywords: multidrug-resistant, poultry, Salmonella spp., tetracycline.

Keywords: multidrug-resistant, poultry, Salmonella spp., tetracycline.

How to cite this article: Waghamare RN, Paturkar AM, Vaidya VM, Zende RJ, Dubal ZN, Dwivedi A, Gaikwad RV (2018) Phenotypic and genotypic drug resistance profile of Salmonella serovars isolated from poultry farm and processing units located in and around Mumbai city, India, Veterinary World, 11(12): 1682-1688.

Received: 26-08-2018 Accepted: 25-10-2018 Published online: 16-12-2018

Corresponding author: R. N. Waghamare E-mail: rupeshwaghmare@gmail.com

DOI: 10.14202/vetworld.2018.1682-1688

Copyright: Waghamare, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.