Anti-inflammatory activity of Jatropha curcas Linn. latex in cream formulation on CD68 expression in mice skin wound

Vet World Vol.11 February-2018 Article-1

Research Article

Veterinary World, 11(2): 99-103

https://doi.org/10.14202/vetworld.2018.99-103

Muhammad Nur Salim¹, Dian Masyitha², Abdul Harris³, Ummu Balqis¹, Cut Dahlia Iskandar², Muhammad Hambal⁴, and Darmawi^5,6

1. Laboratory of Pathology, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh 23111, Indonesia.
2. Laboratory of Histology, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh 23111, Indonesia.
3. Laboratory of Pharmacology, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh 23111, Indonesia.
4. Laboratory of Parasitology, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh 23111, Indonesia.
5. Laboratory of Microbiology, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh 23111, Indonesia.
6. Laboratory of Research, Faculty of Veterinary Medicine, Syiah Kuala University, Darussalam, Banda Aceh 23111, Indonesia.

Background and Aim: The purpose of the present study was to determine the potential of Jatropha curcas latex in the cream formulation on CD68 immune expression (macrophages) during inflammatory phase wound healing process in mice skin.

Materials and Methods: Amount of 12 two-months-old male mice were used between 30 and 40 g. To surgical procedures, wound skin incision was performed 2.0 cm in length until subcutaneous on the paravertebral of each animal. The treatment was carried under locally anesthetized with procaine cream. The mice were allotted into four groups of each, entire surface of each group wound covered by base cream control, sulfadiazine 0.1% cream, J. curcas latex cream 10% and, 15%, respectively. All experiments were performed twice a day for 3 days. The wound healing was assayed in stained histological sections in immunohistochemical of the wounds. CD68 expression was investigated under a microscope.

Results: The results showed that the cream from the 10% and 15% latex of J. curcas revealed moderate immune reaction to CD68 on wound healing.

Conclusion: We concluded that the latex cream of J. curcas possesses anti-inflammatory activity in wound healing process of mice skin. Keywords: anti-inflammatory, CD68, Jatropha curcas latex cream, wound healing.

Keywords: anti-inflammatory, CD68, Jatropha curcas latex cream, wound healing.

How to cite this article: Salim MN, Masyitha D, Harris A, Balqis U, Iskandar CD, Hambal M, Darmawi (2018) Anti-inflammatory activity of Jatropha curcas Linn. latex in cream formulation on CD68 expression in mice skin wound, Veterinary World, 11(2): 99-103.

Received: 12-09-2017 Accepted: 19-12-2017 Published online: 02-02-2018

Corresponding author: Darmawi E-mail: darmawi@unsyiah.ac.id

DOI: 10.14202/vetworld.2018.99-103

Copyright: Salim, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.