Effect of exogenous melatonin and different photoperiods on oxidative status and antioxidant enzyme activity in Chhotanagpuri ewe

Vet World Vol.11 February-2018 Article-6

Research Article

Veterinary World, 11(2): 130-134

https://doi.org/10.14202/vetworld.2018.130-134

Pankaj Kumar Choudhary¹, Ajay Kumar Ishwar², Rajesh Kumar³, Debasish Niyogi⁴, and Mukesh Kumar⁵

1. Department of Veterinary Physiology and Biochemistry, College of Veterinary Science and A.H., N.D.U.A.&T., Kumarganj, Faizabad - 224 229, Uttar Pradesh, India.
2. Department of Veterinary Physiology, Ranchi College of Veterinary Science and A.H., Birsa Agricultural University, Ranchi - 834 006, Jharkhand, India.
3. Department of Agronomy (A.H.), Bihar Agricultural University, Sabour, Bhagalpur, Bihar, India.
4. Department of Veterinary Pathology, College of Veterinary Science and A.H., N.D.U.A.&T., Kumarganj, Faizabad - 224 229, Uttar Pradesh, India.
5. Department of Veterinary Anatomy and Histology, College of Veterinary Science and A.H., N.D.U.A.&T., Kumarganj, Faizabad - 224 229, Uttar Pradesh, India.

Background and Aim: The present study was conducted to evaluate the effect of exogenous melatonin under different photoperiods on oxidative status in Chhotanagpuri ewe.

Materials and Methods: A total of 42 non-pregnant, non-lactating Chhotanagpuri ewe, having body weight ranging between 14.11±0.09 and 15.38±0.06 kg, were selected and were isolated from rams 2 months before melatonin administration. The selected animals were allocated randomly into seven groups, namely, Group I (normal control), Group II (long day [LD] control), Group III (LD+melatonin administration orally, 3 mg/day), Group IV (LD+melatonin administration subcutaneously, 1 mg/day), Group V (short day [SD] control), Group VI (SD+melatonin administration orally, 3 mg/day), and Group VII (SD+melatonin administration subcutaneously, 1 mg/day) comprising six animals in each group. Rams were then introduced into each group after completion of exogenous administration of melatonin. Blood samples with anticoagulant in vials were collected from each animal day before the start of the experiment and thereafter every month up to 5th month. Hemolysate was prepared for estimation of oxidative stress parameters such as malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT).

Results: It was observed that the level of MDA was significantly (p<0.05) higher in LD groups (Group II, III and IV) in comparison to control and SD groups (VI and VII) at 1st month. MDA concentration after exogenous administration of melatonin was significantly (p<0.05) decreased in Group IV and VI in comparison to 1st month. SOD was significantly (p<0.05) higher in SD groups (V, VI, and VII) at the 1st month in comparison to 0 day. After exogenous administration of melatonin, SOD concentration was significantly (p<0.05) higher in Groups III and IV in comparison to 1st month. CAT was significantly (p<0.05) higher in SD groups (V, VI, and VII) in comparison to control and LD groups. After exogenous administration of melatonin, CAT concentration was significantly (p<0.05) higher in Groups III, IV, VI, and VIII in comparison to Groups I, II, and V. At the 3rd month, CAT concentration significantly (p<0.05) decreased in Groups III, IV, VI, and VII in comparison to 2nd month of experiment. However, a decreasing trend of CAT was observed in all the groups from 3rd to 5th month.

Conclusion: The present experiment revealed that exogenous melatonin was able to reduce significantly the level of MDA and increased the activity of SOD and CAT in Chhotanagpuri ewe. Keywords: catalase, Chhotanagpuri ewe, exogenous melatonin, malondialdehyde, photoperiod, superoxide dismutase.

Keywords: catalase, Chhotanagpuri ewe, exogenous melatonin, malondialdehyde, photoperiod, superoxide dismutase.

How to cite this article: Choudhary PK, Ishwar AK, Kumar R, Niyogi D, Kumar M (2018) Effect of exogenous melatonin and different photoperiods on oxidative status and antioxidant enzyme activity in Chhotanagpuri ewe, Veterinary World, 11(2): 130-134.

Received: 29-08-2017 Accepted: 26-12-2017 Published online: 07-02-2018

Corresponding author: Pankaj Kumar Choudhary E-mail: drpankajvet2003@gmail.com

DOI: 10.14202/vetworld.2018.130-134

Copyright: Choudhary, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.