Vet World Vol.11 January-2018 Article-13
Research Article
Veterinary World, 11(1): 71-79
https://doi.org/10.14202/vetworld.2018.71-79
Edible bird's nest impact on rats' uterine histomorphology, expressions of genes of growth factors and proliferating cell nuclear antigen, and oxidative stress level
2. Department of Anatomy and Histology, Faculty of Veterinary Medicine, University of Kufa, Najaf, Iraq.
3. Department of Veterinary Preclinical Sciences, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
4. Department of Community Health, College of Health and Medical Techniques, Al-Furat Al-Awsat Technical University, Iraq.
5. Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Background and Aim: This study aimed to evaluate the effect of edible bird's nest (EBN) supplementation on the uteri of rats based on analyses of the morphological and histomorphometric changes, and expressions of epidermal growth factor (EGF) and its receptor (REGF) genes, vascular endothelial growth factor (VEGF), proliferating cell nuclear antigen (PCNA), and steroid receptors.
Materials and Methods: Twenty-four Sprague Dawley rats were equally distributed into the following four groups: G1 (control), G2, G3, and G4 represented the groups treated with EBN at graded concentrations of 0, 30, 60, and 120 mg/kg body weight (BW) per day for 8 weeks, respectively. During the experimental period, the BW of each rat was recorded weekly. At the proestrus stage of estrous cycle, blood samples were collected from the hearts of anesthetized rats that were later sacrificed. The uteri were removed for histological and immunohistochemical analyses.
Results: The EBN-treated groups showed an increase in the weights and lengths of uteri as compared to the control. Results showed that relative to G1 and G2, G3 and G4 exhibited proliferation in their uterine luminal and glandular epithelia and uterine glands, and up-regulated expressions of EGF, REGF, VEGF, PCNA, and progesterone receptor, and estrogen receptor in their uteri. The EBN increased the antioxidant (AO) and total AO capacities and reduced the oxidative stress (OS) levels in non-pregnant rats.
Conclusion: Findings of this study revealed that EBN promotes proliferation of the uterine structures as evidenced by the upregulation of the expressions of steroid receptors,EGF, REGF, VEGF, and PCNA in the uterus and increased in the plasma concentrations of AO and reduced levels of OS. Keywords: antioxidant, edible bird's nest, growth factors, proliferating cell nuclear antigen, uterus.
Keywords: antioxidant, edible bird's nest, growth factors, proliferating cell nuclear antigen, uterus.
How to cite this article: Albishtue AA, Yimer N, Zakaria MZA, Haron AW, Yusoff R, Assi MA, Almhanawi BH (2018) Edible bird's nest impact on rats' uterine histomorphology, expressions of genes of growth factors and proliferating cell nuclear antigen, and oxidative stress level, Veterinary World, 11(1): 71-79.
Received: 04-09-2017 Accepted: 15-12-2017 Published online: 27-01-2018
Corresponding author: Nurhusien Yimer E-mail: nurdeg2006@gmail.com
DOI: 10.14202/vetworld.2018.71-79
Copyright: Albishtue, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.