Vet World   Vol.11   June-2018  Article-15

Species adulteration in raw milk samples using polymerase chain reaction-restriction fragment length polymorphism

Background and Aim: Milk adulteration is pivotal because it leads to worse effects in public health as human adverse reactions with clinical signs ranged from gastrointestinal signs to anaphylactic shock. This study was carried out to estimate the prevalence of adulteration in buffalo's milk sold in Assiut City, Egypt.

Materials and Methods: A total of 50 raw buffalo's milk samples were collected and examined for adulteration by addition of cow's milk. The examination carried out by applying polymerase chain reaction-restriction fragment length polymorphism technique using cytochrome b (cyt b) gene primers and Hinf I enzymes. The size of target gene was 360 bp in both animal species and amplicon can be digested using Hinf I enzyme, this restriction enzyme divided the essential band to clear three bands at 360, 210, and 150 bp in cows' milk, while, the enzyme could not be cleaved the amplicon in buffalo's samples.

Results: The obtained results cleared that the incidence of adulteration of buffalo's milk very high percentage reaches 90%.

Conclusion: It could be concluded that the raw buffalo's milk sold in Assiut City subject to fraudulent practice and thus can lead to public health hazards. Keywords: buffalo's milk, cyt b gene, Hinf I enzyme, medicolegal, milk adulteration, polymerase chain reaction-restriction fragment length polymorphism.

Keywords: buffalo's milk, cyt b gene, Hinf I enzyme, medicolegal, milk adulteration, polymerase chain reaction-restriction fragment length polymorphism.

How to cite this article: Ewida RM, El-Magiud DSMA (2018) Species adulteration in raw milk samples using polymerase chain reaction-restriction fragment length polymorphism, Veterinary World, 11(6): 830-833.

Received: 09-03-2018  Accepted: 14-05-2018     Published online: 22-06-2018

Corresponding author: Rania M. Ewida   E-mail: r_ewida@aun.edu.eg

DOI: 10.14202/vetworld.2018.830-833

Copyright: Ewida and El-Magiud DSMA, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.