ISMap02 element targeted nested polymerase chain in the detection of Mycobacterium avium subsp. paratuberculosis in fecal samples of cattle and buffaloes

Vet World Vol.11 March-2018 Article-22

Research Article

Veterinary World, 11(3): 397-401

https://doi.org/10.14202/vetworld.2018.397-401

Mamta Rani¹, Deepti Narang¹, Dinesh Kumar¹, Mudit Chandra¹, Sikh Tejinder Singh², and G. Filia³

1. Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India.
2. Directorate of Livestock Farms, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India.
3. Animal Disease Research Centre, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India.

Background and Aim: Johne's disease is chronic granulomatous enteritis which affects ruminants. There are many diagnostic approaches for the detection of Mycobacterium avium subsp. paratuberculosis (MAP) of which molecular detection methods using various elements are less time consuming and more accurate. The present study was conducted using ISMap02 element for nested polymerase chain reaction (nPCR) based detection of MAP in fecal samples. The aim was to test the sensitivity and specificity of the ISMap02 element and also to use this element for the detection of MAP in fecal samples of cattle and buffaloes.

Materials and Methods: A total of 211 fecal samples of cattle and buffaloes from different herds around Ludhiana aged between 2 and 13 years were collected, and DNA extraction was done from these samples. The nPCR was carried out for the detection of MAP in fecal samples.

Results: The ISMap02 element was specific for the detection of MAP only and showed a sensitivity of detection of 7.6 fg/μL of the standard genomic DNA. Among the 211 fecal samples of cattle and buffaloes tested for the ISMap02 element, 18 samples (8.5%) were positive for MAP.

Conclusion: The ISMap02 element is specific and sensitive for the detection of MAP in various samples, and when used in nPCR format, it can increase the sensitivity of detection. Keywords: ISMap02, Mycobacterium avium subsp. paratuberculosis, nested polymerase chain reaction, paratuberculosis.

Keywords: ISMap02, Mycobacterium avium subsp. paratuberculosis, nested polymerase chain reaction, paratuberculosis.

How to cite this article: Rani M, Narang D, Kumar D, Chandra M, Singh ST, Filia G (2018) ISMap02 element targeted nested polymerase chain in the detection of Mycobacterium avium subsp. paratuberculosis in fecal samples of cattle and buffaloes, Veterinary World, 11(3): 397-401.

Received: 29-09-2017 Accepted: 21-02-2018 Published online: 31-03-2018

Corresponding author: Deepti Narang E-mail: deeptivet@rediffmail.com

DOI: 10.14202/vetworld.2018.397-401

Copyright: Rani, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.