Metabolic and morphometric changes in Indonesian cynomolgus monkeys (Macaca fascicularis) fed an atherogenic diet composed of locally sourced ingredients

Vet World Vol.11 November-2018 Article-14

Research Article

Veterinary World, 11(11): 1609-1617

https://doi.org/10.14202/vetworld.2018.1609-1617

Sri Rahmatul Laila¹, Dewi Apri Astuti^2,3, Irma Herawati Suparto^2,4, Ekowati Handharyani⁵, and Dondin Sajuthi⁵

1. Department of Anatomy, Physiology and Pharmacology, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia.
2. Primate Research Center, Bogor Agricultural University, Bogor, Indonesia.
3. Department of Nutrition and Feed Technology, Faculty of Animal Science, Bogor Agricultural University, Bogor, Indonesia.
4. Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Bogor, Indonesia.
5. Primate Research Center, Bogor Agricultural University, Bogor, Indonesia Department of Veterinary Clinic, Reproduction and Pathology, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia.

Background and Aim: This study was designed to determine the effects of a new atherogenic diet formulated at Institut Pertanian Bogor (IPB) (Bogor, Indonesia) on metabolic, morphometric, and carotid artery imaging of cynomolgus monkeys.

Materials and Methods: A total of 20 adult male cynomolgus monkeys fed IPB-1 atherogenic diet for 1 year. Total plasma cholesterol (TPC), high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol, triglycerides, and morphometric measurements were evaluated at baseline and monthly during the study. Carotid plaques and intima-media thickness (IMT) were measured using ultrasonography at baseline and after 8 months of treatment.

Results: This diet increased TPC, LDL, and TPC/HDL ratio and induced carotid atherosclerosis in this model. The TPC, LDL, and TPC/HDL ratio were positively associated; however, HDL was negatively associated with carotid plaques and IMT.

Conclusion: The IPB-1 atherogenic diet formulated with locally and readily available ingredients provides an economically and scientifically feasible monkey model to study atherosclerosis in Indonesia and Southeast Asia. Keywords: atherogenic diet, carotid artery, cynomolgus, ultrasonography.

Keywords: atherogenic diet, carotid artery, cynomolgus, ultrasonography.

How to cite this article: Laila SR, Astuti DA, Suparto IH, Handharyani E, Sajuthi D (2018) Metabolic and morphometric changes in Indonesian cynomolgus monkeys (Macaca fascicularis) fed an atherogenic diet composed of locally sourced ingredients, Veterinary World, 11(11): 1609-1617.

Received: 29-06-2018 Accepted: 10-10-2018 Published online: 22-11-2018

Corresponding author: Sri Rahmatul Laila E-mail: sajuthi@indo.net.id

DOI: 10.14202/vetworld.2018.1609-1617

Copyright: Laila, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.