Research Article | 12 Oct 2018

The relationship between bacterial sources and genotype to the antimicrobial resistance pattern of Burkholderia pseudomallei

Muhammad Abubakar Sadiq1,2, Latiffah Hassan1, Saleha Abdul Aziz1, Zunita Zakaria1, Hassan Ismail Musa1,2, and Maswati Mat Amin3Show more
VETERINARY WORLD | pg no. 1404-1408 | Vol. 11, Issue 10 | DOI: 10.14202/vetworld.2018.1404-1408
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Abstract

          
Background and Aim: This study was designed to determine whether the phenotypic antibiotic resistance pattern of B. pseudomallei is associated with the source of isolates and the genotype.
Materials and Methods: A collection of 111 B. pseudomallei isolates from veterinary cases of melioidosis and the environments (soil and water) were obtained from stock cultures of previous studies and were phylogenetically characterized by multilocus sequence typing (ST). The susceptibility to five antibiotics, namely meropenem (MEM), imipenem, ceftazidime (CAZ), cotrimoxazole (SXT), and co-amoxiclav (AMC), recommended in both acute and eradication phases of melioidosis treatment were tested using minimum inhibitory concentration antibiotics susceptibility test.
Results: Majority of isolates were susceptible to all antibiotics tested while few resistant strains to MEM, SXT, CAZ, and AMC were observed. Statistically significant association was found between resistance to MEM and the veterinary clinical isolates (p<0.05). The likelihood of resistance to MEM was significantly higher among the novel ST 1130 isolates found in veterinary cases as compared to others.
Conclusion: The resistance to MEM and SXT appeared to be higher among veterinary isolates, and the novel ST 1130 was more likely to be resistant to MEM as compared to others. Keywords: animals, antimicrobial, Burkholderia pseudomallei, environmental, resistance, sequence types, veterinary isolates.
          
Keywords: animals, antimicrobial, Burkholderia pseudomallei, environmental, resistance, sequence types, veterinary isolates.