The relationship between bacterial sources and genotype to the antimicrobial resistance pattern of Burkholderia pseudomallei

Vet World Vol.11 October-2018 Article-7

Research Article

Veterinary World, 11(10): 1404-1408

https://doi.org/10.14202/vetworld.2018.1404-1408

Muhammad Abubakar Sadiq^1,2, Latiffah Hassan¹, Saleha Abdul Aziz¹, Zunita Zakaria¹, Hassan Ismail Musa^1,2, and Maswati Mat Amin³

1. Department of Microbiology and Pathology, Faculty of Veterinary Medicine, Universiti Putra Malaysia (UPM), 44300 UPM Serdang, Selangor Darul Ehsan Malaysia.
2. Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, University of Maiduguri, P.M.B 1069 Maiduguri, Borno State Nigeria.
3. Regional Veterinary Laboratory, Bukit Tengah, Peti Surat 63, 14007 Bukit Mertajam, Seberang Perai Tengah, Pulau Pinang, Malaysia.

Background and Aim: This study was designed to determine whether the phenotypic antibiotic resistance pattern of B. pseudomallei is associated with the source of isolates and the genotype.

Materials and Methods: A collection of 111 B. pseudomallei isolates from veterinary cases of melioidosis and the environments (soil and water) were obtained from stock cultures of previous studies and were phylogenetically characterized by multilocus sequence typing (ST). The susceptibility to five antibiotics, namely meropenem (MEM), imipenem, ceftazidime (CAZ), cotrimoxazole (SXT), and co-amoxiclav (AMC), recommended in both acute and eradication phases of melioidosis treatment were tested using minimum inhibitory concentration antibiotics susceptibility test.

Results: Majority of isolates were susceptible to all antibiotics tested while few resistant strains to MEM, SXT, CAZ, and AMC were observed. Statistically significant association was found between resistance to MEM and the veterinary clinical isolates (p<0.05). The likelihood of resistance to MEM was significantly higher among the novel ST 1130 isolates found in veterinary cases as compared to others.

Conclusion: The resistance to MEM and SXT appeared to be higher among veterinary isolates, and the novel ST 1130 was more likely to be resistant to MEM as compared to others. Keywords: animals, antimicrobial, Burkholderia pseudomallei, environmental, resistance, sequence types, veterinary isolates.

Keywords: animals, antimicrobial, Burkholderia pseudomallei, environmental, resistance, sequence types, veterinary isolates.

How to cite this article: Sadiq MA, Hassan L, Aziz SA, Zakaria Z, Musa HI, Amin MM (2018) The relationship between bacterial sources and genotype to the antimicrobial resistance pattern of Burkholderia pseudomallei, Veterinary World, 11(10): 1404-1408.

Received: 14-05-2018 Accepted: 20-08-2018 Published online: 12-10-2018

Corresponding author: Latiffah Hassan E-mail: latiffah@upm.edu.my

DOI: 10.14202/vetworld.2018.1404-1408

Copyright: Sadiq, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.