Vet World Vol.11 September-2018 Article-18
Research Article
Veterinary World, 11(9): 1316-1320
https://doi.org/10.14202/vetworld.2018.1316-1320
Effect of Newcastle disease virus level of infection on embryonic length, embryonic death, and protein profile changes
2. Department of Poultry Production, Faculty of Animal Husbandry, Brawijaya University, Indonesia.
3. Animal Clinic, Faculty of Veterinary Medicine, Brawijaya University, Indonesia.
Background and Aim: Newcastle disease virus (NDV) is an obligate intracellular parasite. Virus can only live on living cells. The embryonated chicken eggs (ECEs) are one of the growth media of virus that is a cheap, easy to do, and accurate for showing patterns of virus change in the host. Higher virus titers indicate the higher number of viruses and more virulent to infect host. This research aimed to investigate the effect of different level of NDV titer infection in ECEs on protein profile, embryonic length, mortality, and pathological change.
Materials and Methods: The study used a completely randomized design of six treatments and seven replications. The treatments were different level of NDV titer infection in allantoic fluid (AF) of 9-11 days ECEs, i.e., P1=20, P2=26, P3=27, P4=28, P5=29, and P6=210 hemagglutination unit (HAU). All samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Data were analyzed using one-way ANOVA with p=0.05 for length of the embryo and descriptive analysis for embryo mortality, pathology change, and protein band.
Results: The result showed that protein profile of NDV-infected ECEs of all different levels is more complex than protein profile of no NDV-infected ECEs. NDV infected of all different levels showed longer size embryo, higher mortality embryo at the first 2 days, and higher occurrence of hemorrhagic in all part of bodies of embryo than those of no NDV infected.
Conclusion: It was concluded that NDV infection of all different level decreased health conditions of chicken embryo of ECEs of 9-11 days old. Different level of NDV infection of ECEs of 9-11 days old showed no significantly different embryo profiles. However, all of the NDV-infected embryos were shorter, death on the 2nd day, and suffered more hemorrhage on all body surfaces than uninfected NDV embryos. Keywords: embryo, Newcastle disease, pathological change, protein, titer, virus.
Keywords: embryo, Newcastle disease, pathological change, protein, titer, virus.
How to cite this article: Qosimah D, Murwani S, Sudjarwo E, Lesmana MA (2018) Effect of Newcastle disease virus level of infection on embryonic length, embryonic death, and protein profile changes, Veterinary World, 11(9): 1316-1320.
Received: 09-04-2018 Accepted: 30-07-2018 Published online: 24-09-2018
Corresponding author: Dahliatul Qosimah E-mail: dahliatulqosimah@gmail.com
DOI: 10.14202/vetworld.2018.1316-1320
Copyright: Qosimah, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.