Vet World Vol.11 September-2018 Article-20
Research Article
Veterinary World, 11(9): 1326-1330
https://doi.org/10.14202/vetworld.2018.1326-1330
First-time serological and molecular detection of Helicobacter pylori in milk from Algerian local-breed cows
2. Department of Microbiology , Central Laboratory of Clinical Biology, University Hospital Center of Nafisa Hamoud (Parnet), Hussein Dey, Algiers, Algeria.
3. Department of Clinical Medicine, Institute of Veterinary Sciences, Tiaret, Algeria.
Background and Aim: The present study was conducted to detect and identify Helicobacter pylori within local cow breeds in the central region of Algeria.
Materials and Methods: Two hundred (n=200) cows from three provinces of the central region of Algeria were studied, between January 2016 and September 2017. Each cow was subject to stool, milk, and blood sampling. Milk and fecal samples were used to detect and identify H. pylori using bacteriology culture method. Blood and milk samples were used to detect H. pylori immunoglobulin G (IgG) antibody using enzyme-linked immunosorbent assay. Polymerase chain reaction was used to confirm the abundance of H. pylori in milk by detecting glmM gene.
Results: Out of 200 sera and 200 milk samples, 12% (24) and 4% (8/200) were positive for the H. pylori IgG antibody. glmM gene was detected in the milk of 13% of cows and was confirmed in all cows presenting IgG in milk.
Conclusion: From the present study, we concluded that the glmM gene is an important marker for detecting H. pylori in milk. Moreover, Algerian local-breed cows are a source of H. pylori and could be responsible for serious zoonosis. Keywords: Algeria, cows, feces, Helicobacter pylori, milk, serum.
Keywords: Algeria, cows, feces, Helicobacter pylori, milk, serum.
How to cite this article: Guessoum M, Guechi Z, Adnane M (2018) First-time serological and molecular detection of Helicobacter pylori in milk from Algerian local-breed cows, Veterinary World, 11(9): 1326-1330.
Received: 18-05-2018 Accepted: 13-08-2018 Published online: 25-09-2018
Corresponding author: Meryem Guessoum E-mail: myriam.guessoum@gmail.com
DOI: 10.14202/vetworld.2018.1326-1330
Copyright: Guessoum, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.