Detection of Toxoplasma gondii copro-prevalence by polymerase chain reaction using repetitive 529 bp gene in feces of pet cats (Felis catus) in Yogyakarta, Indonesia

Vet World Vol.11 September-2018 Article-22

Research Article

Veterinary World, 11(9): 1338-1343

https://doi.org/10.14202/vetworld.2018.1338-1343

Muhammad Hanafiah¹, Joko Prastowo², Sri Hartati³, Dwinna Aliza⁴, and Raden Wisnu Nurcahyo²

1. Parasitology Laboratory, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Indonesia.
2. Department of Parasitology, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, Indonesia.
3. Department of Clinic, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, Indonesia.
4. Pathology Laboratory, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Indonesia.

Background and Aim: The aim of this research was to determine the copro-prevalence of Toxoplasma gondii using polymerase chain reaction (PCR) with repetitive 529 bp gene and to construct the phylogenetic tree of Toxoplasma oocyst from pet cats in Yogyakarta.

Materials and Methods: 9 of 132 pet cat samples which serologically positive for Toxoplasma were used in this research. To determine the copro-prevalence of T. gondii in pet cat, 10 g of feces samples taken from practitioners and household cats in Yogyakarta were used in the PCR method utilizing repetitive 529 bp gene sequences.

Results: The result shows that copro-prevalence by PCR using repetitive 529 bp gene was 33.3% (3/9). The phylogenetic tree of Toxoplasma grouped into two clades, which clade 1 consists of Toxoplasma isolates collected from pet cats in Yogyakarta Indonesia and T. gondii isolates from China and in clade 2 consist of the T. gondii isolates from India.

Conclusion: Copro-prevalence of T. gondii in pet cats in Yogyakarta by means of PCR using repetitive 529 bp gene is around 33.3%. Keywords: copro-prevalence, pet cat, polymerase chain reaction, Toxoplasma gondii.

Keywords: copro-prevalence, pet cat, polymerase chain reaction, Toxoplasma gondii.

How to cite this article: Hanafiah M, Prastowo J, Hartati S, Aliza D, Nurcahyo RW (2018) Detection of Toxoplasma gondii copro-prevalence by polymerase chain reaction using repetitive 529 bp gene in feces of pet cats (Felis catus) in Yogyakarta, Indonesia, Veterinary World, 11(9): 1338-1343.

Received: 26-04-2018 Accepted: 31-07-2018 Published online: 28-09-2018

Corresponding author: Raden Wisnu Nurcahyo E-mail: wisnu-nc@ugm.ac.id

DOI: 10.14202/vetworld.2018.1338-1343

Copyright: Hanafiah, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.