Vet World Vol.11 September-2018 Article-24
Research Article
Veterinary World, 11(9): 1349-1356
https://doi.org/10.14202/vetworld.2018.1349-1356
Effects of secretome on cisplatin-induced testicular dysfunction in rats
2. Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, 55821, Indonesia.
3. Department of Physiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, 55821, Indonesia.
Background and Aim: This study aimed to investigate the effect of secretome derived from the human umbilical MSCs on cisplatin-induced testicular dysfunction in rats.
Materials and Methods: Thirty-six male Wistar rats were divided into the control and secretome-treated groups. In the secretome-treated group, testicular dysfunction was induced by 3 mg/kg BW of cisplatin intraperitoneally 3 times with 3-day intervals. The secretome-treated group was divided according to dose: Low-dose (0.2 mL/kg BW) and high-dose (0.5 mL/kg BW) groups. Secretomes were injected intraperitoneally once a week for 3 weeks. 1 week after the injection of secretome, the cauda epididymis of the rats was removed for spermatozoa evaluation and histological examination.
Results: After the injection of secretome, the sperm motility of the high-dose group showed thin wave-like, rare, and slow movements. No abnormal sperm morphology was observed in all the treated groups. The number of spermatozoa increased gradually in the high-dose group after the injection of secretome. The developmental stages of the spermatogenic cells were complete in both spermatozoa groups after the injection of secretome. However, the spermatozoa in the seminiferous tubules of the high-dose group were denser. Vimentin and cytokeratin immunoreactivities were very strong in the high-dose group 1 week after the second secretome injection.
Conclusion: High-dose secretome derived from the human fetal umbilical cord could increase the number and motility of sperms in rats with cisplatin-induced testicular dysfunction. The administration of high-dose secretome was effective 1 week after the second dose, as indicated by very strong immunoreactivity for vimentin and cytokeratin. Moreover, secretome could promote the regeneration of the seminiferous tubules of both the groups. Keywords: cisplatin, cytokeratin, secretome, spermatogenesis, testicular dysfunction, vimentin.
Keywords: cisplatin, cytokeratin, secretome, spermatogenesis, testicular dysfunction, vimentin.
How to cite this article: Prihatno SA, Padeta I, Larasati AD, Sundari B, Hidayati A, Fibrianto YH, Budipitojo T (2018) Effects of secretome on cisplatin-induced testicular dysfunction in rats, Veterinary World, 11(9): 1349-1356.
Received: 18-05-2018 Accepted: 16-08-2018 Published online: 29-09-2018
Corresponding author: Teguh Budipitojo E-mail: budipitojo@ugm.ac.id
DOI: 10.14202/vetworld.2018.1349-1356
Copyright: Prihatno, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.