The neuroprotective effect of Ocimum sanctum Linn. ethanolic extract on human embryonic kidney-293 cells as in vitro model of neurodegenerative disease

Vet World Vol.11 September-2018 Article-6

Research Article

Veterinary World, 11(9): 1237-1243

https://doi.org/10.14202/vetworld.2018.1237-1243

Puspa Hening¹, Made Bagus Auriva Mataram², Nastiti Wijayanti³, Dwi Liliek Kusindarta², and Hevi Wihadmadyatami²

1. Research Center of Biotechnology, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia.
2. Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia.
3. Department of Animal Physiology, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia.

Background and Aim: This study aimed to analyze the neuroprotective effect of Ocimum sanctum Linn. ethanolic extract (OSE) on human embryonic kidney-293 (HEK-293) cells as the in vitro model of neurodegenerative diseases.

Materials and Methods: In this research, HEK-293 cells divided into five groups consisting of normal and healthy cells (NT), cells treated with Camptothecin 500 μM as the negative control, cells treated with trimethyltin 10 μM (TMT), cells treated with OSE 75 μg/ml, and cells pre-treated with OSE 75 μg/ml then induced by TMT 10 μM (OSE+TMT). MTT assay and phase contrast microscopy were applied to observe the cell viability quantitatively and morphological after Ocimum sanctum Linn extract treatment. Finally, the reverse transcription polymerase chain reaction was employed to study the expression of choline acetyltransferase (ChAT).

Results: The MTT assay and phase contrast microscopy showed that OSE pre-treatment significantly increased the viability of TMT-induced apoptotic cells and maintained cell viability of the normal HEK-293 cells. Expression of ChAT markedly reduced on TMT treatment group, but OSE administration stabilized ChAT expression in TMT-induced HEK-293 cells.

Conclusion: This present study proved that OSE administration has neuroprotective effect by increased HEK-293 cells viability and maintain ChAT expression. Keywords: choline acetyltransferase, human embryonic kidney-293, neurodegenerative diseases, Ocimum sanctum Linn. ethanolic extract.

Keywords: choline acetyltransferase, human embryonic kidney-293, neurodegenerative diseases, Ocimum sanctum Linn. ethanolic extract.

How to cite this article: Hening P, Mataram MBA, Wijayanti N, Kusindarta DL, Wihadmadyatami H (2018) The neuroprotective effect of Ocimum sanctum Linn. ethanolic extract on human embryonic kidney-293 cells as in vitro model of neurodegenerative disease, Veterinary World 11(9): 1237-1243.

Received: 13-05-2018 Accepted: 24-07-2018 Published online: 11-09-2018

Corresponding author: Hevi Wihadmadyatami E-mail: heviwihadmadyatami@ugm.ac.id

DOI: 10.14202/vetworld.2018.1237-1243

Copyright: Hening, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.