Vet World Vol.12 April-2019 Article-1
Research Article
Veterinary World, 12(4): 477-482
https://doi.org/10.14202/vetworld.2019.477-482
Sumateran wild boar (Sus scrofa vittatus) meat antibody production as immunodiagnostic reagent candidate
2. Indonesia Agricultural Quarantine Agency, Indonesia.
3. Department of Animal Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, Bogor Agricultural University, Indonesia.
4. Center of Veterinary Research, Bogor, Indonesia.
5. Department of Animal Husbandry, Faculty of Animal Husbandry, State Polytechnic of Lampung, Indonesia.
Background and Aim: Meat authentication gives significance values in view of religious, food safety, public health, quality assurance, and legal concern. Most of the meat authentication is based on molecular assay; a simpler method to authenticate meat is needed to develop. An immunoassays technique may offer a solution for simpler test. The aim of our current study was to develop a polyclonal antibody of Sus scrofa vittatus (Sumateran wild boar) as an immunodiagnostic reagent candidate.
Materials and Methods: Three male New Zealand white rabbits were used in this study for antibody production. Antigen used was meat extract of Sumateran wild boar, each rabbit was immunized with meat extract antigen (0.5 mg/ml) emulsified in Freund's complete adjuvant at a 1:1 (v/v) ratio as much as 1 ml at subcutaneous route. Booster was carried out 3 times with interval time of 14 days, using meat extract antigen emulsified in Freund's incomplete adjuvant at a 1:1 (v/v) ratio. Serum samples were taken every week, start from 1 week after the first immunization up to 1 week after the third booster. Antibody purification was performed using ammonium sulfate precipitation and Protein A. The presence of specific antibody was determined using agar gel precipitation test and enzyme-linked immunosorbent assay, while purified specific IgG was characterized using sodium dodecyl sulfate-polyacrylamide gel electrophoresis method.
Results: Specific antibody was detected at 14 days after the first immunization and still detected until 2 weeks after the third booster. Highest absorbance of specific antibody was detected 1 week after the third booster.
Conclusion: The present study demonstrated that specific antibody of Sumateran wild boar is favorable to be produced in rabbit and showed that antibody produced is applicable to detect Sumateran wild boar meat antigen in immunodiffusion assay, indicating that it is promising as a reagent candidate in immunodiagnostic assay/kit. Keywords: antibody, enzyme-linked immunosorbent assay, rabbit, reagent, Sumateran wild boar.
Keywords: antibody, enzyme-linked immunosorbent assay, rabbit, reagent, Sumateran wild boar.
How to cite this article: Adiningsih MW, Soejoedono RD, Adji RS, Putri DD, Purnawarman T, Latif H, Poetri ON (2019) Sumateran wild boar (Sus scrofa vittatus) meat antibody production as immunodiagnostic reagent candidate. Veterinary World, 12(4): 477-482.
Received: 17-10-2018 Accepted: 14-02-2019 Published online: 01-04-2019
Corresponding author: Retno Damajanti Soejoedono E-mail: retnodmail@yahoo.com
DOI: 10.14202/vetworld.2019.477-482
Copyright: Adiningsih, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.