Vet World Vol.12 April-2019 Article-18
Research Article
Veterinary World, 12(4): 605-608
https://doi.org/10.14202/vetworld.2019.605-608
Molecular detection and phylogenetic tree of infectious laryngotracheitis virus in layers in Al-Diwaniyah province, Iraq
2. Department of Internal and Preventive Medicine, College of Veterinary Medicine, University of Al-Qadisiyah, Diwaniyah, Iraq.
Background and Aim: Infectious laryngotracheitis (ILT) of chickens is a substantial issue to be studied in Iraq because this disease is one of the most highly contagious respiratory diseases in the world caused by a herpesvirus. However, in Iraq, the ILT virus (ILTV) infection and disease have yet not been confirmed in layers, so farm owners do not vaccinate these layers. The current study aimed to document the detection and characterization of ILTV in layer hens from Al-Diwaniyah city, for the first time in Iraq, using molecular techniques like polymerase chain reaction (PCR) and sequencing.
Materials and Methods: Four layer farms (15,000 unvaccinated layers/farm) in Al-Diwaniyah province, Iraq, suffered a severe ILT outbreak, was diagnosed and reported by clinical and PCR tests. This disease has been reported in Iraq, and more recently, it began to show outbreaks in Al-Diwaniyah city. The current work opted to investigate the ILTV using PCR and DNA sequencing techniques. The study targeted the p32 gene of ILTV using pooled tracheal swabs and organs including the trachea, lung, and kidneys which were collected from dead and clinically infected chickens.
Results: The analyses revealed that four of six suspected field samples showed positive results by PCR. The DNA sequencing results showed the homology of the amplified fragments with the studied gene.
Conclusion: This study confirmed the presence of ILTV in hens with respiratory signs during the outbreak. Keywords: DNA sequencing, infectious laryngotracheitis virus, laryngotracheitis, laying hens.
Keywords: DNA sequencing, infectious laryngotracheitis virus, laryngotracheitis, laying hens.
How to cite this article: Alaraji F, Hammadi H, Abed AA, Khudhair YI (2019) Molecular detection and phylogenetic tree of infectious laryngotracheitis virus in layers in Al-Diwaniyah province, Iraq, Veterinar World, 12(4): 605-608.
Received: 20-11-2018 Accepted: 11-03-2019 Published online: 25-04-2019
Corresponding author: Furkan Alaraji E-mail: furkan.sabbar@qu.edu.iq
DOI: 10.14202/vetworld.2019.605-608
Copyright: Alaraji, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.