Vet World Vol.12 August-2019 Article-2
Research Article
Veterinary World, 12(8): 1180-1187
https://doi.org/10.14202/vetworld.2019.1180-1187
Seroprevalence and molecular characterization of Mycobacterium bovis infection in camels (Camelus dromedarius) in the Delta region, Egypt
2. Department of Animal Hygiene and Zoonoses, Faculty of Veterinary Medicine, The New Valley University, Egypt.
3. Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Mansoura University, Egypt.
4. Tuberculosis Unit, Animal Health Research Institute, Dokki, Egypt.
5. Department of Meat Hygiene, Faculty of Veterinary Medicine, The New Valley University, Egypt.
6. Directorate of Veterinary Medicine, Behira Governorate, Egypt.
Background and Aim: This study aimed to determine the prevalence rates of Mycobacterium infection in camel sera collected before slaughter and gross lesion tissue collected at postmortem (PM) using enzyme-linked immunosorbent assay (ELISA), bacteriological culture, and polymerase chain reaction (PCR). In addition, serum samples from humans who had occupational contact with camels were tested by ELISA and sputum sample by culture.
Materials and Methods: ELISA was performed on serum samples antemortem. In addition, bacteriological culture and PCR were conducted after PM. Tuberculosis infection was identified in humans who had contact with camels using ELISA for serum samples and culture for sputum samples.
Results: Tuberculous lesions were detected in 184 of 10,903 camels (1.7%). The ELISA results revealed that of the 184 examined camel serum samples, 124 (67.39%) were positive and all 20 camel serum samples that had no associated tuberculous lesions were negative. Moreover, only one of 48 (2.08%) human serum samples was positive by ELISA. Mycobacterial culture revealed 112 isolates from the 184 examined camel samples (60.87%), while human sputum sample cultures were all negative. PCR analysis identified the mpb70 gene in three of seven randomly tested samples.
Conclusion: Gene sequencing was performed on two samples and the sequences were submitted to the National Center for Biotechnology Information GenBank (accession numbers MF990289 and MG59479). A phylogenetic tree was constructed based on the partial DNA sequences of the mpb70 gene; the similarity between the isolates was 98.1%. The similarities between the two isolates and the standard strains of Mycobacterium bovis in GenBank were 98.1% and 100%, respectively. Further investigation on the antemortem detection of M. bovis infection in camels is needed to decrease public risk. Keywords: camel, enzyme-linked immunosorbent assay, Mycobacterium bovis, polymerase chain reaction, tuberculosis.
Keywords: camel, enzyme-linked immunosorbent assay, Mycobacterium bovis, polymerase chain reaction, tuberculosis.
How to cite this article: Elnaker YF, Diab MS, Ibrahim NA, El-Gedawy A, Zaki RS, Radwan A (2019) Seroprevalence and molecular characterization of Mycobacterium bovis infection in camels (Camelus dromedarius) in the Delta region, Egypt, Veterinary World, 12(8): 1180-1187.
Received: 12-04-2019 Accepted: 21-06-2019 Published online: 05-08-2019
Corresponding author: Mohmed S. Diab E-mail: mohameddiab333@gmail.com
DOI: 10.14202/vetworld.2019.1180-1187
Copyright: Elnaker, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.