Vet World Vol.12 December-2019 Article-11
Research Article
Veterinary World, 12(12): 1959-1965
https://doi.org/10.14202/vetworld.2019.1959-1965
An in vitro and in silico evaluation of the antibacterial activity of the bioactive compounds in Majapahit (Crescentia cujete L.) fruit
2. Department of Biology Education, Faculty of Teacher Training and Education, University of PGRI Ronggolawe, Tuban 62381, East Java, Indonesia.
Background and Aim: Majapahit (Crescentia cujete L.) fruit extract acts as a natural antibacterial agent due to its bioactive constituents such as tannins, flavonoids, triterpenoids, and saponins. The aim of this study was to determine the antibacterial activity of Majapahit fruit against Vibrio harveyi both in vitro and in silico.
Materials and Methods: Column chromatography, minimum inhibitory concentration (MIC) determination, and transmission electron microscopy (TEM) were used for in vitro analysis. In silico analysis was performed using PubChem® database, Pass Online (Way2Drug.com©), Search Tool 17 Interacting Chemicals (STITCH), and UNIPROT database (https://www.uniprot.org/).
Results: The MIC was found to be 0.313 mg/mL. Within the concentration range of 0.313 mg/mL-10 mg/mL, Majapahit fruit extract could inhibit the growth of V. harveyi, while lower concentrations of 0.078 mg/mL and 0.165 mg/mL indicated the presence of bacterial growth. The pathogenic mechanism of V. harveyi on vannamei shrimp (Litopenaeus vannamei) involved targeting cytochrome P450, cyclin-dependent kinase 6, and caspases 3 and 8. This was indicated by cell damage observed through TEM.
Conclusion: This study provides comprehensive results on the potential of Majapahit fruit as a natural antibacterial agent. Thus, Majapahit fruit can be considered for functional food applications. Keywords: antibacterial, Crescentia cujete, in silico, in vitro, Majapahit fruit.
Keywords: antibacterial, Crescentia cujete, in silico, in vitro, Majapahit fruit.
How to cite this article: Rahmaningsih S, Pujiastutik H (2019) An
Received: 22-07-2019 Accepted: 29-10-2019 Published online: 13-12-2019
Corresponding author: Sri Rahmaningsih E-mail: rahmaningsihsri@unirow.ac.id
DOI: 10.14202/vetworld.2019.1959-1965
Copyright: Rahmaningsih and Pujiastutik, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.