Effectiveness of six molecular typing methods as epidemiological tools for the study of Salmonella isolates in two Colombian regions

Vet World Vol.12 December-2019 Article-17

Research Article

Veterinary World, 12(12): 1998-2006

https://doi.org/10.14202/vetworld.2019.1998-2006

Kelly Lozano-Villegas¹, Roy Rodríguez-Hernández², and Iang Rondón-Barragán^1,2

1. Research Group in Immunobiology and Pathogenesis, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Santa Helena Highs, Ibagué 730006299, Colombia.
2. Poultry Research Group, Faculty of Veterinary Medicine, University of Tolima, Santa Helena Highs, Ibagué 730006299, Colombia.

Background and Aim: The aim of this study was the genotypic characterization of the strains of Salmonella spp. isolated from broiler chickens and humans with gastroenteritis from two regions of Colombia, by BOXA1R-polymerase chain reaction (PCR) and random amplification of polymorphic DNA (RAPD)-PCR methods.

Materials and Methods: Forty-nine strains of Salmonella were assessed, 15 from poultry farms in Santander region, and 34 from Tolima region isolated from poultry farms (n=24) and the stool samples of people with gastroenteritis (n=10). BOXA1R primers were selected for repetitive element-based PCR (REP-PCR) and five arbitrary primers, namely, GTG 5, OPB 15, OPP 16, OPS 11, and P 1254 were used for RAPD-PCR to generate DNA fingerprints from the isolates. Fingerprint data from each typing method were under composite analysis and the diversity of the data was analyzed by grouping (clustering). The dendrogram was generated by the unweighted group method with analysis of the arithmetic mean based on the Dice similarity coefficient. In addition, Simpson's index was evaluated to discriminate the power of the methods.

Results: OPP 16 primer and composite analysis proved to be superior compared to other REP-PCR typing methods. The best discriminatory index was observed when GTG 5 (0.92) and OPP 16 (0.85) primers were used alone or combined with RAPD-PCR and BOX-PCR (0.99).

Conclusion: This study indicated that OPP 16 and GTG 5 primers provide suitable molecular typing results for the discrimination of the genetic relationship among Salmonella spp. isolates and may be useful for epidemiological studies. Keywords: dendrogram, serotyping, typing methods.

Keywords: dendrogram, serotyping, typing methods.

How to cite this article: Lozano-Villegas K, Rodríguez-Hernández R, Rondón-Barragán I (2019) Effectiveness of six molecular typing methods as epidemiological tools for the study of

Received: 20-09-2019 Accepted: 20-11-2019 Published online: 19-12-2019

Corresponding author: Iang Rondón-Barragán E-mail: isrondon@ut.edu.co

DOI: 10.14202/vetworld.2019.1998-2006

Copyright: Lozano-Villegas, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.