Vet World Vol.12 February-2019 Article-22
Research Article
Veterinary World, 12(2): 331-336
https://doi.org/10.14202/vetworld.2019.331-336
Isolation and molecular characterization of Mannheimia haemolytica and Pasteurella multocida associated with pneumonia of goats in Chhattisgarh
2. Department of Veterinary Public Health and Epidemiology, College of Veterinary Science and Animal Husbandry, Anjora, Durg, Chhattisgarh, India.
3. Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Anjora, Durg, Chhattisgarh, India.
Background and Aim: The purpose of this study was to isolate and characterize the Mannheimia haemolytica and Pasteurella multocida from blood, nasal discharge, and lung tissue of pneumonic goats.
Materials and Methods: A total of 14 goats were investigated for pneumonic pasteurellosis. Of 14 goats, nasal swabs and blood samples were collected from 10 clinically diseased animals. Moreover, lung tissue and heart blood samples were collected during necropsy of four goats died with pneumonia. All the samples were processed for the isolation of M. haemolytica and P. multocida in the laboratory. Bacterial isolates were identified by cultural and biochemical characters and 16S rRNA sequence analysis. All the isolates were subjected to susceptibility testing using commonly used antimicrobials. M. haemolytica isolates were characterized by PHSSA gene detection. P. multocida isolates were characterized by KMT1 gene detection and capsule typing.
Results: On necropsy of dead goats, the pneumonia was characterized as acute fibrinous bronchopneumonia. Bacterial culture revealed the isolation of M. haemolytica (7) and P. multocida (5) of 10 clinical cases. Moreover, M. haemolytica and P. multocida were coisolated from two of the lung tissues. Furthermore, one of the other two lung tissues showed the isolation of M. haemolytica while the other showed recovery of P. multocida. Bacterial isolates were specifically identified by the 16S rRNA sequence analysis. The isolates showed reduced susceptibility to β-lactams, aminoglycosides, and fluoroquinolones . Moreover, the PHSSA and KMT1 genes were specifically detected among M. haemolytica, and P. multocida isolates, respectively. All P. multocida isolates belonged to serogroup A.
Conclusion: The present study reported an occurrence of pneumonic pasteurellosis caused by M. haemolytica and P. multocida in a goat flock. Keywords: bronchopneumonia, goat, Mannheimia haemolytica, Pasteurella multocida, pneumonic pasteurellosis.
Keywords: bronchopneumonia, goat, Mannheimia haemolytica, Pasteurella multocida, pneumonic pasteurellosis.
How to cite this article: Rawat N, Gilhare VR, Kushwaha KK, Hattimare DD, Khan FF, Shende RK, Jolhe DK (2019) Isolation and molecular characterization of Mannheimia haemolytica and Pasteurella multocida associated with pneumonia of goats in Chhattisgarh, Veterinary World, 12(2): 331-336.
Received: 06-07-2018 Accepted: 02-01-2019 Published online: 24-02-2019
Corresponding author: Nidhi Rawat E-mail: dr_nidhirawat@yahoo.com
DOI: 10.14202/vetworld.2019.331-336
Copyright: Rawat, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.