Association of single nucleotide polymorphisms in the fatty acid synthase, LOC514211, and fat mass and obesity-associated genes with milk traits in Indonesian-Holstein dairy cattle

Vet World Vol.12 July-2019 Article-35

Research Article

Veterinary World, 12(7): 1160-1166

https://doi.org/10.14202/vetworld.2019.1160-1166

Amalia Puji Rahayu^1,2, Tety Hartatik³, Agung Purnomoadi¹, and Edy Kurnianto¹

1. Department of Animal Science, Faculty of Animal and Agricultural Sciences, Diponegoro University, Semarang 50275, Indonesia.
2. Agriculture, Fisheries and Food Center of Semarang Regency, Ungaran 50514, Indonesia.
3. Department of Animal Breeding and Reproduction, Faculty of Animal Sciences, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.

Background and Aim: The study aimed to identify fatty acid synthase (FASN), LOC514211, and fat mass and obesity-associated (FTO) gene polymorphisms and to investigate their associations with milk traits in an Indonesian-Holstein dairy cow population.

Materials and Methods: A total of 100 Indonesian-Holstein cows consisting of 50 heads (0th generation; G0) and 50 heads of their daughters (1st generation; G1) were used. Polymerase chain reaction-restriction fragment length polymorphism was performed to genotype three single nucleotide polymorphisms: rs41919985 in the FASN gene, rs42688595 in the LOC514211 gene, and g.1371T>A in the FTO gene.

Results: FASN rs41919985 was associated with milk protein percentage (p<0.05), FTO g.1371T>A was associated with milk fat percentage (p<0.05), and LOC514211 rs42688595 was not associated with any trait (p>0.05). Heterozygote variants showed a higher protein percentage for FASN and the highest fat percentage for FTO. These associations were consistent in the G0 and G1 populations.

Conclusion: Our results indicate that the milk protein and fat percentages can be improved by increasing the frequency of the AG genotype of FASN and the AT genotype of FTO, respectively. Keywords: fat mass and obesity-associated, fatty acid synthase, Indonesian-Holstein cattle, LOC514211, milk traits.

Keywords: fat mass and obesity-associated, fatty acid synthase, Indonesian-Holstein cattle, LOC514211, milk traits.

How to cite this article: Rahayu AP, Hartatik T, Purnomoadi A, Kurnianto E (2019) Association of single nucleotide polymorphisms in the fatty acid synthase, LOC514211, and fat mass and obesity-associated genes with milk traits in Indonesian-Holstein dairy cattle, Veterinary World, 12(7): 1160-1166.

Received: 13-05-2019 Accepted: 25-06-2019 Published online: 29-07-2019

Corresponding author: Edy Kurnianto E-mail: kurniantoedy17@gmail.com

DOI: 10.14202/vetworld.2019.1160-1166

Copyright: Rahayu, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.