Vet World Vol.12 July-2019 Article-5
Research Article
Veterinary World, 12(7): 959-964
https://doi.org/10.14202/vetworld.2019.959-964
Sequence analysis of the cytochrome c oxidase subunit 1 gene of Sarcoptes scabiei isolated from goats and rabbits in East Java, Indonesia
2. Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga, Surabaya, Indonesia.
3. Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
4. Study Program of Animal Health, Faculty of Vocational, Universitas Airlangga, Surabaya, Indonesia.
5. Master Program Student, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
Background and Aim: This study aimed to sequence the Cytochrome c oxidase (COX-1) gene sequence from mitochondrial DNA of Sarcoptes scabiei isolated from Lamongan goats and Mojokerto rabbits, align it with DNA isolated from Zi'gong rabbit (GenBank accession No. EU256389.1), and produce a phylogenetic analysis of S. scabiei COX-1 gene.
Materials and Methods: S. scabiei mites were obtained from goats and rabbits, and DNA was extracted using QIAamp DNA Mini Kit. The forward and reverse primer sequences were designed based on the DNA sequence of an S. scabiei COX-1 gene isolated from the Zi'gong rabbit (5'-TCTTAGGGGCTGGATTTAGTATG-3' and 5'-AGTTCCTCTACCAGTTCCAC-3', respectively). To confirm sequencing output, the sequence resulting from the reverse primer was inverted and aligned to the sequence from the forward primer using Clone Manager Professional Version 9 for Windows (Scientific & Educational Software; http://www.scied.com). This alignment was subsequently used to build a phylogenetic tree, using the Neighbor- Joining method, in the MEGA6 program (https://www.megasoftware.net/).
Results: Polymerase chain reaction (PCR) products from S. scabiei isolates from Lamongan goats and Mojokerto rabbits produced bands of around 290 bp with 2% agarose gel electrophoresis. Comparing the DNA sequences of the S. scabiei COX-1 gene with those isolated from Lamongan goats and Mojokerto rabbits showed 99% homology.
Conclusion: PCR products of the S. scabiei COX-1 gene isolated from Lamongan goats and Mojokerto rabbits were around 290 bp long. The sequences had more than 99% homology. The sequences of the COX-1 gene of S. scabiei from Lamongan goats and Mojokerto rabbits were relatively close to the sequence of the gene in S. scabiei obtained from various hosts according to National Center for Biotechnology Information data. Keywords: cytochrome c oxidase-1, East Java, goat, Indonesia, rabbit, Sarcoptes scabiei.
Keywords: cytochrome c oxidase-1, East Java, goat, Indonesia, rabbit, Sarcoptes scabiei.
How to cite this article: Lastuti NDR, Rohman A, Handiyatno D, Chrismanto D, Desiandura K (2019) Sequence analysis of the cytochrome c oxidase subunit 1 gene of Sarcoptes scabiei isolated from goats and rabbits in East Java, Indonesia, Veterinary World, 12(7): 959-964.
Received: 23-01-2019 Accepted: 15-05-2019 Published online: 05-07-2019
Corresponding author: Nunuk Dyah Retno Lastuti E-mail: nunukdyah53@gmail.com
DOI: 10.14202/vetworld.2019.959-964
Copyright: Lastuti NDR, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.