Vet World Vol.12 June-2019 Article-13
Research Article
Veterinary World, 12(6): 809-816
https://doi.org/10.14202/vetworld.2019.809-816
In vivo approach on femur bone regeneration of white rat (Rattus norvegicus) with the use of hydroxyapatite from cuttlefish bone (Sepia spp.) as bone filler
2. Program Study of Biomedical Engineering, Department of Physics, Faculty of Science and Technology, Universitas Airlangga, Surbaya, East Java 60115, Indonesia.
3. Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya, East Java 60115, Indonesia.
Background and Aim: This study aimed to determine femur bone regeneration of white rats (Rattus norvegicus) through the use of HA from cuttlefish bone (Sepia spp.) as bone filler.
Materials and Methods: HA was made using the hydrothermal method by mixing 1M aragonite (CaCO3) from cuttlefish bone and 0.6 M NH4H2PO4 at 200°C for 12 h followed by sintering at 900°C for 1 h. In vivo test was carried out in three groups, including control group, bovine bone-derived HA group, and cuttlefish bone-derived HA group. The generation of femur bone was observed through the number of osteoblasts, osteoclasts, woven bone, lamellar bone, havers system, and repair bone through anatomical pathology test for 28 days and 56 days.
Results: Anatomical pathology test results are showed that administration of bovine bone-derived HA and cuttlefish bone-derived HA increased the number of osteoblasts, osteoclasts, woven bone, lamellar bone, havers system, and bone repair at recuperation of 56 days. Statistical test using Statistical Package for the Social Sciences with Kruskal–Wallis and Mann–Whitney U-test was resulted in significant differences between the bovine bone-derived HA control group and the cuttlefish-derived HA control group. There was no significant difference toward the indication of bone formation through the growth of osteoblasts, osteoclasts, woven bone, lamellar bone, havers system, and bone repair in the bovine bone-derived HA and cuttlefish bone-derived HA groups.
Conclusion: It can be concluded that cuttlefish bone-derived HA has the potential as bone filler based on the characteristics of bone regeneration through in vivo test. Keywords: anatomical pathology, bone filler, cuttlefish bone (Sepia spp.), hydrothermal, hydroxyapatite.
Keywords: anatomical pathology, bone filler, cuttlefish bone (Sepia spp.), hydrothermal, hydroxyapatite.
How to cite this article: Aminatun A, Handayani DEF, Widiyanti P, Winarni D, Siswanto S (2019) In vivo approach on femur bone regeneration of white rat (Rattus norvegicus) with the use of hydroxyapatite from cuttlefish bone (Sepia spp.) as bone filler, Veterinary World, 12(6): 809-816.
Received: 17-12-2018 Accepted: 18-04-2019 Published online: 14-06-2019
Corresponding author: Aminatun Aminatun E-mail: aminatun@fst.unair.ac.id
DOI: 10.14202/vetworld.2019.809-816
Copyright: Aminatun, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.