A sensitive serodiagnostic tool for the detection of active infection of zoonotic visceral and nasopharyngeal linguatulosis

Vet World Vol.12 June-2019 Article-24

Research Article

Veterinary World, 12(6): 883-889

https://doi.org/10.14202/vetworld.2019.883-889

Marwa M. Attia¹, Elshaimaa Ismael², and Nagla M. K. Saleh³

1. Department of Parasitology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.
2. Department of Veterinary Hygiene and Management, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.
3. Department of Zoology, Faculty of Science, Aswan University, Aswan, Egypt.

Background and Aim: This study aimed to evaluate the different serological techniques for early diagnosis of acute concurrent infections with linguatulosis in the definitive host (dogs) and an intermediate host (goats). This evaluation compared between the gold standard (GS) test (GS; examination of nasal and fecal samples in dogs and examination of lymph nodes in goats), sandwich enzyme-linked immunosorbent assay (S-ELISA), and indirect ELISA.

Materials and Methods: Fifty goats and fifty dogs were examined for the presence of Linguatula serrata nymphs and adults, respectively, besides the collection of blood samples from the examined animals for serologic testing.

Results: In goats; GS, S-ELISA, and indirect ELISA showed positivity in 32 (64%), 28 (56%), and 39 (78%) samples, respectively. In dogs; GS, S-ELISA, and indirect ELISA showed positivity in 25 (50%), 25 (50%), and 30 (60%) samples, respectively. S-ELISA displayed significant higher agreement with the GS test (≥0.83) than indirect ELISA (≤0.67) in both hosts. Infection with linguatulosis showed significant relation with the age of goats and dogs and the sex of goats (p<0.05).

Conclusion: S-ELISA displayed more sensitivity and specificity for the detection of concurrent infections with linguatulosis in both dogs and goats than indirect ELISA, which could detect the prior infections. Similarly, these assays could be used for diagnosis of concurrent infections with linguatulosis in human, especially the chronic ones. Keywords: halzoun, indirect enzyme-linked immunosorbent assay, Linguatula serrata, sandwich enzyme-linked immunosorbent assay, visceral linguatulosis.

Keywords: halzoun, indirect enzyme-linked immunosorbent assay, Linguatula serrata, sandwich enzyme-linked immunosorbent assay, visceral linguatulosis.

How to cite this article: Attia MM, Ismael E, Saleh NMK (2019) A sensitive serodiagnostic tool for the detection of active infection of zoonotic visceral and nasopharyngeal linguatulosis, Veterinary World, 12(6): 883-889.

Received: 19-12-2018 Accepted: 10-05-2019 Published online: 24-06-2019

Corresponding author: Marwa M. Attia E-mail: marwaattia.vetpara@yahoo.com

DOI: 10.14202/vetworld.2019.883-889

Copyright: Attia, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.