Occurrence, characterization, and antibiogram of Staphylococcus aureus in meat, meat products, and some seafood from Libyan retail markets

Vet World Vol.12 June-2019 Article-30

Research Article

Veterinary World, 12(6): 925-931

https://doi.org/10.14202/vetworld.2019.925-931

Hesham T. Naas¹, Ramadan A. Edarhoby¹, Aboubaker M. Garbaj¹, Salah M. Azwai², Said K. Abolghait³, Fatim T. Gammoudi², Ashraf A. Moawad⁴, Ilaria Barbieri⁵, and Ibrahim M. Eldaghayes²

1. Department of Food Hygiene and Control, Faculty of Veterinary Medicine, University of Tripoli, P.O. Box 13662, Tripoli, Libya.
2. Department of Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Tripoli, P.O. Box 13662, Tripoli, Libya.
3. Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt.
4. Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Cairo University, 12211 Giza 12211, Egypt.
5. Department of Genetics, The Lombardy and Emilia Romagna Experimental Zootechnic Institute, Via Bianchi 9, Brescia 25124, Italy.

Background and Aim: The aim of the current investigation was to screen the presence of Staphylococci spp., especially S. aureus in meat, meat products of different animal species, and some seafood sold in some retail markets in Libya using cultural and molecular techniques, and to study their antibiotics resistance profiles.

Materials and Methods: A total of 139 samples from red meat, meat products, and seafood were collected from many areas in Libya. Enumeration and isolation of Staphylococci spp. and S. aureus by normal cultural methods followed by molecular identification using molecular techniques by bacterial DNA extraction and partial sequencing of 16S rDNA.

Results: Out of 139 samples, 112 (80.6%) were contaminated with different species of Staphylococci based on cultural characteristics of Staphylococci on Baird-Parker medium, for which S. aureus was detected in only 32 samples (23%). However, only six out of 18 (33.3%) isolates sent for sequencing were confirmed to be S. aureus using the molecular technique. The six identified isolates of S. aureus were tested for antimicrobial resistance against 24 most commonly used antibiotics. All isolates were resistant to only two antibiotics (cefotaxime and clindamycin). Among these six isolates, only one confirmed to be Methicillin-resistant Staphylococcus aureus.

Conclusion: Results of this study suggest that food of animal origin could be a source of S. aureus with antimicrobial resistance characteristics that can be spread through the food chain, and raise the importance of these results for public health. Keywords: 16S rDNA, antibiogram, meat products, meat, seafood, Staphylococcus aureus.

Keywords: 16S rDNA, antibiogram, meat products, meat, seafood, Staphylococcus aureus.

How to cite this article: Naas HT, Edarhoby RA, Garbaj AM, Azwai SM, Abolghait SK, Gammoudi FT, Moawad AA, Barbieri I, Eldaghayes IM (2019) Occurrence, characterization, and antibiogram of Staphylococcus aureus in meat, meat products, and some seafood from Libyan retail markets, Veterinary World, 12(6): 925-931.

Received: 07-03-2019 Accepted: 13-05-2019 Published online: 29-06-2019

Corresponding author: Ibrahim M. Eldaghayes E-mail: ibrahim.eldaghayes@vetmed.edu.ly

DOI: 10.14202/vetworld.2019.925-931

Copyright: Naas, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.