Characterization and comparison of strains of Pasteurella multocida associated with cases of progressive atrophic rhinitis and porcine pneumonia in Argentina

Vet World Vol.12 March-2019 Article-13

Research Article

Veterinary World, 12(3): 434-439

https://doi.org/10.14202/vetworld.2019.434-439

Fernando A. Bessone¹, Maria Laura Soriano Perez^1,2, Gustavo Zielinski¹, Marina Dibarbora^1,2, M. B. Conde¹, Javier Cappuccio^1,2, and Fabrisio Alustiza¹

1. Department of Animal Health, Instituto Nacional de Tecnologia Agropecuaria, Estacion Experimental Agropecuaria Marcos Juarez, Marcos Juarez, Cordoba, Argentina.
2. CONICET, Buenos Aires, Argentina.

Background and Aim: This study aimed to characterize the Pm strains recovered from clinical cases of PN and PAR and to elucidate the antibiotic susceptibility profiles of the strains.

Materials and Methods: Sixty strains were characterized molecularly by polymerase chain reaction to determine species-specific gene, capsular type (A or D), and toxin A production. The agar diffusion method was employed to evaluate antibiotic resistance profiles.

Results: We found that 65% of strains belonged to capsular type A or D, and 15% of those were positive to toxA gene. The antibiotic susceptibility profiles found were sensitive in decreasing order to: Enrofloxacin, ceftiofur (CTF), ampicillin, tilmicosin (TIL), florfenicol (FFN), spectinomycin (SPC), gentamicin, oxytetracycline (OTC), and trimethoprim-sulfamethoxazole (TMS). Strains were resistant in decreasing order to: Lincomycin (LIN), tylosin (TYL), erythromycin (ERY), TMS, SPC, OTC, FFN, TIL, and CTF.

Conclusion: The toxA gene was detected in many Pm isolates from pneumonic lungs. Capsule type A or D was the most frequently found among the collected isolates. LIN, TYL, and ERY are the drugs which showed higher percentages of resistant isolates. Keywords: antibiotics susceptibility profiles, molecular characterization, Pasteurella multocida, pig.

Keywords: antibiotics susceptibility profiles, molecular characterization, Pasteurella multocida, pig.

How to cite this article: Bessone FA, Perez MLS, Zielinski G, Dibarbora M, Conde MB, Cappuccio J, Alustiza F (2019) Characterization and comparison of strains of Pasteurella multocida associated with cases of progressive atrophic rhinitis and porcine pneumonia in Argentina, Veterinary World, 12(3): 434-439.

Received: 25-06-2018 Accepted: 06-02-2019 Published online: 21-03-2019

Corresponding author: Fernando A. Bessone E-mail: bessone.fernando@inta.gob.ar

DOI: 10.14202/vetworld.2019.434-439

Copyright: Bessone, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.