Vet World   Vol.12   March-2019  Article-13

Research Article

Veterinary World, 12(3): 434-439

https://doi.org/10.14202/vetworld.2019.434-439

Characterization and comparison of strains of Pasteurella multocida associated with cases of progressive atrophic rhinitis and porcine pneumonia in Argentina

Fernando A. Bessone1, Maria Laura Soriano Perez1,2, Gustavo Zielinski1, Marina Dibarbora1,2, M. B. Conde1, Javier Cappuccio1,2, and Fabrisio Alustiza1
1. Department of Animal Health, Instituto Nacional de Tecnologia Agropecuaria, Estacion Experimental Agropecuaria Marcos Juarez, Marcos Juarez, Cordoba, Argentina.
2. CONICET, Buenos Aires, Argentina.

Background and Aim: This study aimed to characterize the Pm strains recovered from clinical cases of PN and PAR and to elucidate the antibiotic susceptibility profiles of the strains.

Materials and Methods: Sixty strains were characterized molecularly by polymerase chain reaction to determine species-specific gene, capsular type (A or D), and toxin A production. The agar diffusion method was employed to evaluate antibiotic resistance profiles.

Results: We found that 65% of strains belonged to capsular type A or D, and 15% of those were positive to toxA gene. The antibiotic susceptibility profiles found were sensitive in decreasing order to: Enrofloxacin, ceftiofur (CTF), ampicillin, tilmicosin (TIL), florfenicol (FFN), spectinomycin (SPC), gentamicin, oxytetracycline (OTC), and trimethoprim-sulfamethoxazole (TMS). Strains were resistant in decreasing order to: Lincomycin (LIN), tylosin (TYL), erythromycin (ERY), TMS, SPC, OTC, FFN, TIL, and CTF.

Conclusion: The toxA gene was detected in many Pm isolates from pneumonic lungs. Capsule type A or D was the most frequently found among the collected isolates. LIN, TYL, and ERY are the drugs which showed higher percentages of resistant isolates. Keywords: antibiotics susceptibility profiles, molecular characterization, Pasteurella multocida, pig.

Keywords: antibiotics susceptibility profiles, molecular characterization, Pasteurella multocida, pig.

How to cite this article: Bessone FA, Perez MLS, Zielinski G, Dibarbora M, Conde MB, Cappuccio J, Alustiza F (2019) Characterization and comparison of strains of Pasteurella multocida associated with cases of progressive atrophic rhinitis and porcine pneumonia in Argentina, Veterinary World, 12(3): 434-439.

Received: 25-06-2018  Accepted: 06-02-2019     Published online: 21-03-2019

Corresponding author: Fernando A. Bessone   E-mail: bessone.fernando@inta.gob.ar

DOI: 10.14202/vetworld.2019.434-439

Copyright: Bessone, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.