Antiseptic effect of natural teat dip containing lactic acid against mastitis-causing Escherichia coli

Vet World Vol.12 March-2019 Article-8

Research Article

Veterinary World, 12(3): 397-401

https://doi.org/10.14202/vetworld.2019.397-401

Rinrada Chotigarpa¹, Kannika Na Lampang¹, Surachai Pikulkaew^2,3, Siriporn Okonogi^4,3, Pirote Silman⁵, and Raktham Mektrirat^1,3

1. Department of Veterinary Biosciences and Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand.
2. Department of Food Animal Clinic, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand.
3. Research Center of Pharmaceutical Nanotechnology, Chiang Mai University, Chiang Mai 50200, Thailand.
4. Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand.
5. Faculty of Animal Science and Technology, Maejo University, Chiang Mai 50290, Thailand.

Background and Aim: This study aimed to estimate the enumeration of total bacteria and coliform on teat skin from dairy cows and evaluate the efficacy of the natural rice gel containing 5% v/v lactic acid (NGL) against Escherichia coli standard and field strains isolated from bovine teat skin.

Materials and Methods: A total of 100 bacterial teat skin samples (25 cows) were collected from dairy cows in smallholder farm. The cows were housed in freestall barns. The colonization of total bacteria and E. coli on teat skin was measured by 3M Petrifilm method. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of lactic acid were evaluated for reference strain of E. coli ATCC 25922 and two field strains of E. coli. The natural teat sanitizer was formulated using 5% NGL with modified rice gel. In vitro antiseptic efficacy of 5% NGL was determined by time-kill kinetic assay. E. coli morphology after exposure with 5% NGL was examined under a scanning electron microscope (SEM).

Results: The total bacteria and coliform counts from bovine teat skin were 2.11×104 and 1.54×101 colony-forming units/ml, respectively. The MIC and MBC of lactic acid on the tested bacteria were 0.5% v/v. The natural teat dip was successfully prepared with minimum change in consistency after 1 year of storage at 4°C. The reduction rate of 5% NGL on E. coli ATCC 25922 and field strain showed 32.77% and 27.58%, respectively. An appearance under SEM of non-viable E. coli after being incubated with 5% NGL clearly showed atypical form and rough surface cell membrane.

Conclusion: The rice gel containing 5% v/v lactic acid is a promising preparation as a natural teat antiseptic for reducing bacteria on teat skin. It was shown to be effective against E. coli causing bovine mastitis in dairy cows. Keywords: antiseptic, Escherichia coli, lactic acid, teat skin.

Keywords: antiseptic, Escherichia coli, lactic acid, teat skin.

How to cite this article: Chotigarpa R, Na Lampang K, Pikulkaew S, Okonogi S, Silman P, Mektrirat R (2019) Antiseptic effect of natural teat dip containing lactic acid against mastitis-causing Escherichia coli, Veterinary World, 12(3): 397-401.

Received: 01-09-2018 Accepted: 04-02-2019 Published online: 15-03-2019

Corresponding author: Raktham Mektrirat E-mail: raktham.m@cmu.ac.th

DOI: 10.14202/vetworld.2019.397-401

Copyright: Chotigarpa, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.