Antibiotic-resistant Escherichia coli in deer and nearby water sources at Safari parks in Bangladesh

Vet World Vol.12 October-2019 Article-10

Research Article

Veterinary World, 12(10): 1578-1583

https://doi.org/10.14202/vetworld.2019.1578-1583

Md Samun Sarker¹, Abdul Ahad¹, Saurav Kumar Ghosh¹, Md Shahriar Mannan², Arup Sen¹, Sirazul Islam³, Md Bayzid⁴, and Zamila Bueaza Bupasha¹

1. Department of Microbiology and Veterinary Public Health, Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Chattogram 4225, Bangladesh.
2. Department of Livestock Services, Upazila Livestock Office, Thakurgaon Sadar, Thakurgaon, Bangladesh.
3. Department of Livestock Services, Upazila Livestock Office, Mithamain, Kishoreganj, Bangladesh.
4. Department of Pathology and Parasitology, Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Chattogram 4225, Bangladesh.

Background and Aim: The emergence and rapid dissemination of multidrug-resistant (MDR) bacteria in different ecosystems is a growing concern to human health, animal health, and the environment in recent years. The study aimed to determine the antibiotic resistance in Escherichia coli from deer and nearby water sources at two different Safari parks in Bangladesh.

Materials and Methods: A number of 55 fresh fecal samples of deer and six water samples from nearby lakes were collected from two Safari parks. Samples were processed, cultured, and carried out biochemical tests for E. coli. The antibiotic susceptibility was determined by disk diffusion method. To identify the resistance genes, polymerase chain reaction was performed.

Results: A total of 32 E. coli isolates from 55 fecal samples and 6 of 6 E. coli isolates from lake water were isolated. From fecal E. coli isolates, ampicillin and sulfamethoxazole were 90.63% (n=29/32) resistant and 87.5% (n=28/32) were resistant to tetracycline and nalidixic acid. High resistance was also observed to other antibiotics. On the contrary, all E. coli isolates from water sources were 100% (n=6/6) resistant to ampicillin, tetracycline, sulfamethoxazole, and nalidixic acid. MDR was revealed in all water samples, whereas 96.88% (n=31/32) was found in fecal isolates. A number of blaTEM, tetA, and Sul2 genes were detected from both isolates.

Conclusion: This study for the 1st time highlights, a significant proportion of E. coli isolates in wildlife deer and nearby water sources were MDR in Bangladesh. Keywords: antibiotic-resistant, deer, Escherichia coli, lake, multidrug-resistant.

Keywords: antibiotic-resistant, deer, Escherichia coli, lake, multidrug-resistant.

How to cite this article: Sarker MS, Ahad A, Ghosh SK, Mannan MS, Sen A, Islam S, Bayzid M, Bupasha ZB (2019) Antibiotic-resistant Escherichia coli in deer and nearby water sources at Safari parks in Bangladesh, Veterinary World, 12(10): 1578-1583.

Received: 17-05-2019 Accepted: 03-09-2019 Published online: 19-10-2019

Corresponding author: Md Samun Sarker E-mail: samuncvasu@gmail.com

DOI: 10.14202/vetworld.2019.1578-1583

Copyright: Sarker, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.