Vet World   Vol.12   October-2019  Article-15

Research Article

Veterinary World, 12(10): 1616-1623

https://doi.org/10.14202/vetworld.2019.1616-1623

The potency of chitosan-based Pinus merkusii bark extract nanoparticles as anti-cancer on HeLa cell lines

Annise Proboningrat1, Amaq Fadholly1, Regina Purnama Dewi Iskandar2, Agung Budianto Achmad3, Fedik Abdul Rantam4, and Sri Agus Sudjarwo5
1. Doctoral Student, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia.
2. Doctoral Student, Faculty of Medicine, Universitas Airlangga, Surabaya 60115, Indonesia.
3. Department of Health, Faculty of Vocational Studies, Universitas Airlangga, Surabaya 60115, Indonesia.
4. Department of Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia.
5. Department of Pharmacology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia.

Background and Aim: Cervical cancer accounts for the fourth as a cause of death from cancer in women worldwide, with more than 85% of events and deaths occurring in developing countries. The main problems of chemotherapy are the lack of selectivity and drug resistance. This study aimed to investigate the signal transduction of chitosan-based Pinus merkusii bark extract nanoparticles (Nano-PMBE) as an anticancer on HeLa cell line.

Materials and Methods: Nano-PMBE was prepared based on the ionic gelation method. Its anticancer activities in HeLa cells were investigated through cytotoxicity test, cell cycle, and apoptosis analysis. The expression of p53 and caspase-9 was also observed.

Results: The results showed that Nano-PMBE has a size of 394.3 nm. Meanwhile, the Nano-PMBE was cytotoxic to HeLa cells ( IC50 of 384.10 μg/ml), caused G0/G1 phase arrest and cell apoptosis in HeLa cells. Besides, the expression of p53 and caspase-9 has increased.

Conclusion: The results showed a notable anticancer effect of Nano-PMBE by arresting the cell cycle and inducing apoptosis in HeLa cells, suggesting that it might have therapeutic potential for cervical cancer. Further research is needed to find out more about the anticancer mechanism of Nano-PMBE in HeLa cells to in vivo and clinical studies. Keywords: chitosan, HeLa cells, nanoparticles, Pinus merkusii.

Keywords: chitosan, HeLa cells, nanoparticles, Pinus merkusii.

How to cite this article: Proboningrat A, Fadholly A, Iskandar RPD, Achmad AB, Rantam FA, Sudjarwo SA (2019) The potency of chitosan-based Pinus merkusii bark extract nanoparticles as anti-cancer on HeLa cell lines, Veterinary World, 12(10): 1616-1623.

Received: 12-06-2019  Accepted: 05-09-2019     Published online: 25-10-2019

Corresponding author: Sri Agus Sudjarwo   E-mail: ags158@yahoo.com

DOI: 10.14202/vetworld.2019.1616-1623

Copyright: Proboningrat, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.