Vet World Vol.12 October-2019 Article-3
Research Article
Veterinary World, 12(10): 1529-1534
https://doi.org/10.14202/vetworld.2019.1529-1534
Immune response evaluation in Balb/c mice after crude extract of Anisakis typica sensitization
2. Fish Quarantine and Inspection Agency of Kupang, Jalan Ade Irma No 6 Walikota, Kupang 85228, East Nusa Tenggara, Indonesia.
3. Department of Food Processing Technology, Faculty of Fisheries and Marine Sciences, Brawijaya University, Jalan Veteran, Malang 65145, East Java, Indonesia.
4. Department of Biochemistry, Faculty of Sciences, Brawijaya University, Jalan Veteran, Malang 65145, East Java, Indonesia.
5. Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Brawijaya University, Jalan Veteran, Malang 65145, East Java, Indonesia.
Background and Aim: Anisakis is a global challenge for a fish product which may lead to a decrease in economic value and consumers' preference. Skipjack (Katsuwonus pelamis) in Kupang, Nusa Tenggara Timur, Indonesia, have important economic value for local fisheries. Anisakis typica is one of the Anisakis species which potent to induce an allergic reaction. However, the study about A. typica involved in the dendritic cells (DCs), T helper 1 (Th1), T helper 2 (Th2), and regulatory T cells (Tregs) is still limited. This study aimed to analyze the dynamic changed of the immune system including DCs, CD4+ T cells, and Tregs after 1 week of A. typica sensitization.
Materials and Methods: Twenty-four male Balb/C mice were randomly divided into four groups (n=6), mice treated with crude A. typica extract (CAE) 50, 75, and 100 mg/kg BW, respectively. CAE was given orally per day for a week. At the end of the experiment, the animals were sacrificed and the spleen was collected. DCs were labeled as CD11c+ interleukin-6+ (IL-6+); CD4+ T cells were distinguished as Th1 (CD4+ interferon-γ+ [IFN-γ+]) and Th2 (CD4+ IL-4+ and CD4+ IL-5+); Tregs were labeled as CD4+CD25+CD62L+. The expression of each cell was determined by flow cytometry.
Results: Our result described that CAE elicits CD11c+ IL-6+, CD4+ IFN-γ+, CD4+ IL-4+, and CD4+ IL-5+ and reduces CD4+CD25+CD62L+ significantly (p<0.05) in dose-dependent manner in mice after A. typica infection.
Conclusion: The Th1/Th2 ratio after A. typica crude extract treatment exhibits a mixed pattern rather than the classical model allergy to food antigens. Our study is expected as a basic understanding of the changes in immune response after A. typica infection. Keywords: allergy, dendritic cells, inflammation, nematode, regulatory T cells.
Keywords: allergy, dendritic cells, inflammation, nematode, regulatory T cells.
How to cite this article: Haryadi L, Suprayitno E, Aulanni'am A, Hariati AM (2019) Immune response evaluation in Balb/c mice after crude extract of Anisakis typica sensitization, Veterinary World, 12(10): 1529-1534.
Received: 12-06-2019 Accepted: 27-08-2019 Published online: 04-10-2019
Corresponding author: Anik Martinah Hariati E-mail: a_hariati@ub.ac.id
DOI: 10.14202/vetworld.2019.1529-1534
Copyright: Haryadi, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.