Vet World   Vol.12   October-2019  Article-5

Research Article

Veterinary World, 12(10): 1540-1545

https://doi.org/10.14202/vetworld.2019.1540-1545

Identification of Staphylococcus species isolated from preputium of Aceh cattle based on 16S rRNA gene sequences analysis

Muhammad Hambal1, Masda Admi2,3, Safika Safika4, Wahyu Eka Sari5,6, Teuku Reza Ferasyi6, Dasrul Dasrul7, Ummu Balqis8, and Darmawi Darmawi2,9
1. Laboratory of Parasitology, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, Aceh, Indonesia.
2. Laboratory of Microbiology, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, Aceh, Indonesia.
3. Department of Mathematics and Applied Sciences, Universitas Syiah Kuala, Darussalam, Banda Aceh, 23111, Indonesia.
4. Department of Veterinary Infectious Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University, Jl. Agatis Dramaga, Bogor, 16680, West Java, Indonesia.
5. Laboratory of Research, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, Aceh, Indonesia.
6. Laboratory of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, Aceh, Indonesia.
7. Laboratory of Reproduction, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, Aceh, Indonesia.
8. Laboratory of Pathology, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, Aceh, Indonesia.
9. Laboratory of Biomedical Science, Faculty of Public Health, University of Teuku Umar, Meulaboh, Aceh, Indonesia.

Background and Aim: This research aimed to identify Staphylococcus species isolated from preputial swabs of healthy Aceh cattle, based on 16S ribosomal RNA gene analysis.

Materials and Methods: The bacterium was isolated from preputial swabs of healthy Aceh cattle. The total DNA from the isolated bacteria was extracted using the Genomic DNA Mini Kit followed by polymerase chain reaction (PCR) amplification of the 16S rRNA gene. The product of PCR amplification was then sequenced and aligned to the known sequences in the GenBank database by multiple alignments and was also analyzed by bioinformatics software to construct a phylogenetic tree.

Results: The results revealed that the bacterial isolate 3A had genetically closed relation to Staphylococcus pasteuri with <97% maximum identity. Data derived from the phylogenetic tree revealed that the bacterial isolate 3A was also related to Staphylococcus warneri, yet, it shows a different evolutionary distance with the ancestors (S. pasteuri).

Conclusion: The results of this research suggested that the bacterium 3A, isolated from preputial swabs of healthy Aceh cattle, is a Staphylococcus species. Keywords: 16S rRNA gene, Aceh cattle, phylogenetic tree, polymerase chain reaction, Staphylococcus pasteuri.

Keywords: 16S rRNA gene, Aceh cattle, phylogenetic tree, polymerase chain reaction, Staphylococcus pasteuri.

How to cite this article: Hambal M, Admi M, Safika S, Sari WE, Ferasyi TR, Dasrul D, Balqis U, Darmawi D (2019) Identification of Staphylococcus species isolated from preputium of Aceh cattle based on 16S rRNA gene sequences analysis, Veterinary World, 12(10): 1540-1545.

Received: 04-03-2019  Accepted: 29-08-2019     Published online: 08-10-2019

Corresponding author: Darmawi Darmawi   E-mail: darmawi@unsyiah.ac.id

DOI: 10.14202/vetworld.2019.1540-1545

Copyright: Hambal, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.