Vet World Vol.12 October-2019 Article-5
Research Article
Veterinary World, 12(10): 1540-1545
https://doi.org/10.14202/vetworld.2019.1540-1545
Identification of Staphylococcus species isolated from preputium of Aceh cattle based on 16S rRNA gene sequences analysis
2. Laboratory of Microbiology, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, Aceh, Indonesia.
3. Department of Mathematics and Applied Sciences, Universitas Syiah Kuala, Darussalam, Banda Aceh, 23111, Indonesia.
4. Department of Veterinary Infectious Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University, Jl. Agatis Dramaga, Bogor, 16680, West Java, Indonesia.
5. Laboratory of Research, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, Aceh, Indonesia.
6. Laboratory of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, Aceh, Indonesia.
7. Laboratory of Reproduction, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, Aceh, Indonesia.
8. Laboratory of Pathology, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, Aceh, Indonesia.
9. Laboratory of Biomedical Science, Faculty of Public Health, University of Teuku Umar, Meulaboh, Aceh, Indonesia.
Background and Aim: This research aimed to identify Staphylococcus species isolated from preputial swabs of healthy Aceh cattle, based on 16S ribosomal RNA gene analysis.
Materials and Methods: The bacterium was isolated from preputial swabs of healthy Aceh cattle. The total DNA from the isolated bacteria was extracted using the Genomic DNA Mini Kit followed by polymerase chain reaction (PCR) amplification of the 16S rRNA gene. The product of PCR amplification was then sequenced and aligned to the known sequences in the GenBank database by multiple alignments and was also analyzed by bioinformatics software to construct a phylogenetic tree.
Results: The results revealed that the bacterial isolate 3A had genetically closed relation to Staphylococcus pasteuri with <97% maximum identity. Data derived from the phylogenetic tree revealed that the bacterial isolate 3A was also related to Staphylococcus warneri, yet, it shows a different evolutionary distance with the ancestors (S. pasteuri).
Conclusion: The results of this research suggested that the bacterium 3A, isolated from preputial swabs of healthy Aceh cattle, is a Staphylococcus species. Keywords: 16S rRNA gene, Aceh cattle, phylogenetic tree, polymerase chain reaction, Staphylococcus pasteuri.
Keywords: 16S rRNA gene, Aceh cattle, phylogenetic tree, polymerase chain reaction, Staphylococcus pasteuri.
How to cite this article: Hambal M, Admi M, Safika S, Sari WE, Ferasyi TR, Dasrul D, Balqis U, Darmawi D (2019) Identification of Staphylococcus species isolated from preputium of Aceh cattle based on 16S rRNA gene sequences analysis, Veterinary World, 12(10): 1540-1545.
Received: 04-03-2019 Accepted: 29-08-2019 Published online: 08-10-2019
Corresponding author: Darmawi Darmawi E-mail: darmawi@unsyiah.ac.id
DOI: 10.14202/vetworld.2019.1540-1545
Copyright: Hambal, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.