Vet World Vol.12 September-2019 Article-15
Research Article
Veterinary World, 12(9): 1454-1459
https://doi.org/10.14202/vetworld.2019.1454-1459
Prevalence and species identification of Cryptosporidium spp. in the newborn dairy calves from Muang District, Khon Kaen Province, Thailand
2. Division of Pathobiology, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
3. Department of Microbiology and Parasitology, Faculty of Medical Sciences, Phayao University, Phayao 56000, Thailand.
Background and Aim: This study aims to determine the prevalence of Cryptosporidium spp. infection and to identify the species of Cryptosporidium spp. in newborn dairy calves between December 2016 and March 2017 in Muang District, Khon Kaen Province, Thailand.
Materials and Methods: A total of 200 fecal samples from newborn dairy calves of the ages 1 day up to 28 days were collected and the presence of Cryptosporidium oocysts was examined microscopically using the modified Kinyoun's acid-fast staining technique. Then, Cryptosporidium species were identified using nested polymerase chain reaction amplification of 18S rRNA gene and sequencing.
Results: The modified Kinyoun's acid-fast staining revealed the presence of Cryptosporidium oocysts in 51% (102/200). Sequence analysis of the 18S rRNA gene identified two species, namely, Cryptosporidium bovis (n=11) and Cryptosporidium ryanae (n=11) and one isolated strain could not be identified.
Conclusion: This study indicated that newborn dairy calves aging up to 4 weeks were highly infected with Cryptosporidium spp., and the infection mostly occurred in diarrheic dairy calves. This is the first report of Cryptosporidium in dairy calves in Khon Kaen Province and the results provide baseline information for further studies and control of Cryptosporidium infection in dairy calves in the study area. Keywords: Cryptosporidium, Khon Kaen Province, nested polymerase chain reaction, newborn dairy calves, prevalence.
Keywords: Cryptosporidium, Khon Kaen Province, nested polymerase chain reaction, newborn dairy calves, prevalence.
How to cite this article: Doungmala P, Phuektes P, Taweenan W, Sangmaneedet S, Japa O (2019) Prevalence and species identification of Cryptosporidium spp. in the newborn dairy calves from Muang District, Khon Kaen Province, Thailand, Veterinary World, 12(9): 1454-1459.
Received: 04-06-2019 Accepted: 20-08-2019 Published online: 23-09-2019
Corresponding author: Phennarin Doungmala E-mail: phennarin.d@gmail.com
DOI: 10.14202/vetworld.2019.1454-1459
Copyright: Doungmala, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.