Vet World Vol.12 September-2019 Article-2
Research Article
Veterinary World, 12(9): 1362-1371
https://doi.org/10.14202/vetworld.2019.1362-1371
Administration of Streptococcus bovis isolated from sheep rumen digesta on rumen function and physiology as evaluated in a rumen simulation technique system
2. Department of Health and Biological Sciences, Symbiosis International (Deemed University), Pune, Maharashtra, India.
Background and Aim: Little information about the stability and changes of sheep ruminal microbiota due to pathogen intervention in the rumen simulation technique (RUSITEC) is available. This study aimed to investigate the effect of administration of a novel isolated Streptococcus bovis strain on rumen microbiology and physiology. In addition, the isolation of pigment-producing Streptococcus lutetiensis is described.
Materials and Methods: Microbial strains were isolated from sheep rumen digesta. An isolated strain of S. bovis was evaluated in the RUSITEC system fed with mixed cattle feed and compared with an in-house developed probiotic formulation (PF), PF 1, containing Bacillus amyloliquifaciens, Bacillus subtilis, and Propionibacterium freudenreichii. The parameters of volatile fatty acid, lactic acid, pH profiling, and the coliform anti-pathogenicity were evaluated to determine the effect of S. bovis on rumen function and physiology.
Results: Administration of S. bovis reduced the coliform count by 31.20% from 7.2×1010 colony-forming units (CFU)/ mLto 1.7×106 CFU/mL. Agar diffusion assays revealed the extracellular antimicrobial activity of S. bovis against coliforms; Escherichia coli and Salmonella enterica with 12 and 14 mm zones of inhibition, respectively. Simultaneously, an increase of 61.62% in the rumen yeast count was noted. The physiological changes resulted in a 5% reduction in acetic acid concentration from 431 to 405 mg/L.
Conclusion: The present research indicates that S. bovis is highly capable of altering rumen physiology and function on colonization and is a key transition microbe to be studied during rumen intervention studies. A decrease in the coliform count could be attributed to extracellular production of a bacteriocin-like substance, as illustrated through agar diffusion assays. Keywords: bacteriocin, probiotic, rumen simulation technique, rumen, Streptococcus bovis.
Keywords: bacteriocin, probiotic, rumen simulation technique, rumen, Streptococcus bovis.
How to cite this article: Aphale D, Natu A, Laldas S, Kulkarni A (2019) Administration of Streptococcus bovis isolated from sheep rumen digesta on rumen function and physiology as evaluated in a rumen simulation technique system, Veterinary World, 12(9): 1362-1371.
Received: 02-04-2019 Accepted: 22-07-2019 Published online: 11-09-2019
Corresponding author: Aarohi Kulkarni E-mail: aarohikulkarni@praj.net
DOI: 10.14202/vetworld.2019.1362-1371
Copyright: Aphale, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.