Vet World Vol.12 September-2019 Article-22
Research Article
Veterinary World, 12(9): 1499-1505
https://doi.org/10.14202/vetworld.2019.1499-1505
Proteomics analysis of serum protein patterns in duck during aflatoxin B1 exposure
2. Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand.
3. Proteomics Research Laboratory, National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Klongluang, Pathumthani 12120, Thailand.
Background and Aim: Unlike the already well-documented human serum proteome, there are still limitations regarding analyzing and interpreting the various physiological changes and disease states of the serum proteomes found in duck. Serum proteome in duck under the condition of mycotoxin contamination in feed has not yet been examined. This study aimed to introduce the characterization of the circulating proteomes in duck serum during exposure to aflatoxin B1 (AFB1).
Materials and Methods: Duck serum samples were collected from four experimental groups, gel-based mass spectrometry was then applied, and finally, 445 proteins were identified in pulled serum sample.
Results: Among these 445 proteins, 377 were present in at least one group from all. There were 35 proteins which were expressed when the duck was exposed to AFB1. The protein library that allows the identification of a large number of different proteins in duck serum will be enhanced by the addition of these peptide spectral data. It is noteworthy that chromodomain-helicase-DNA-binding protein 7 (CHD7) [Gallus gallus] was up-regulated in the group with the highest AFB1 contamination.
Conclusion: CHD7 protein might be somehow relative to aflatoxicosis in the duck that causes poor performance and economic loss. Moreover, other proteins present in duck serum were also added in the protein library. Keywords: aflatoxin B1, duck, proteomics, serum.
Keywords: aflatoxin B1, duck, proteomics, serum.
How to cite this article: Tansakul N, Rattanasrisomporn J, Roytrakul S (2019) Proteomics analysis of serum protein patterns in duck during aflatoxin B1 exposure, Veterinary World, 12(9): 1499-1505.
Received: 01-07-2019 Accepted: 16-08-2019 Published online: 29-09-2019
Corresponding author: Natthasit Tansakul E-mail: natthasitt@yahoo.com
DOI: 10.14202/vetworld.2019.1499-1505
Copyright: Tansakul, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.