Vet World Vol.12 September-2019 Article-3
Research Article
Veterinary World, 12(9): 1372-1377
https://doi.org/10.14202/vetworld.2019.1372-1377
Identification of carbon nanotube particles in liver tissue and its effects on apoptosis of birds exposed to air pollution
2. Department of Biology, College of Education for Pure Sciences, Wasit University, Wasit, Iraq.
3. Department of Anatomy and Histology, College of Veterinary Medicine, Al-Qasim Green University, Babylon, Iraq.
Background and Aim: This study aimed to distinguish carbon nanotube (CNT) particles and their pathological effects on the liver of birds in areas with carbon emissions.
Materials and Methods: Twenty-one domestic ducks were collected from pure farmers and exposed to different sources of air pollution. Histological stains were used to detect the accumulation of carbon particles. In addition, acridine orange/ ethidium bromide staining was used to detect apoptosis, and scanning electron microscope (SEM) technique was used to determine the morphological design of carbon particles.
Results: Light microscope results showed that the liver sections contain multiwalled CNTs (MWCNTs) which appear as black spots in the hepatic parenchyma. The histopathological changes of parenchyma include sinusoidal dilatation, infiltration, and congestion with frequently high number of macrophages. In general, early destruction of hepatic parenchyma was observed. Moreover, SEM results showed two morphological types of CNTs: The ball-shaped nanoparticles scattered as ultrafine carbon black and fiber form of carbon particles were recognized as MWCNTs in the hepatic tissue. Fluorescence microscopy results showed the early and progressive stages of apoptosis in the hepatic cells of birds in polluted areas, which can be related to the degree and exposure period to pollutants.
Conclusion: The study indicates that liver morbidity of birds living in the farms affected by the pollution of brick factories is higher than the birds living in farms affected by the pollution of oil fields. Keywords: air pollution, apoptosis, carbon nanotube.
Keywords: air pollution, apoptosis, carbon nanotube.
How to cite this article: Al-Badri AM, Bargooth AF, Al-Jebori JG, Zegyer EAK (2019) Identification of carbon nanotube particles in liver tissue and its effects on apoptosis of birds exposed to air pollution, Veterinary World, 12(9): 1372-1377.
Received: 14-05-2019 Accepted: 22-07-2019 Published online: 12-09-2019
Corresponding author: Ahmed Mahdi Al-Badri E-mail: ahmedalmyahi@yahoo.com
DOI: 10.14202/vetworld.2019.1372-1377
Copyright: Al-Badri, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.