Molecular identification of Salmonella Typhimurium from village chickens based on invA and spvC genes

Vet World Vol.13 April-2020 Article-22

Research Article

Veterinary World, 13(4): 764-767

https://doi.org/10.14202/vetworld.2020.764-767

Mwanaisha Mkangara^1,2, Ernest R. Mbega¹, and Musa Chacha¹

1. Department of Sustainable Agriculture and Biodiversity and Ecosystems Management, Nelson Mandela African Institution of Science and Technology, Arusha, Tanzania.
2. Department of Science and Laboratory Technology, Dar es Salaam Institute of Technology, Dar es Salaam, Tanzania.

Background and Aim: This study aimed to identify Salmonella enterica serovars by polymerase chain reaction (PCR) based on virulence genes invasion A (inv A) and Salmonella plasmid virulence C (spvC).

Materials and Methods: DNA extraction of eight bacteria isolates was done using the PowerSoil® DNA Isolation Kit. The amplification of invA and spvC genes was done using conventional PCR. The positive PCR products were purified using the GeneJET Purification Kit and then sequenced using ABI 3730 XL automated genetic analyzer. The sequences obtained were compared for similarities with other Salmonella serovars deposited on the NCBI GenBank using BLASTN.

Results: Four out of eight samples were amplified by primers FS139/RS141 that target invA gene with products of about 284 bp, and three out of four of the same invA positive samples were also amplified by primers FSPV-1/RSPV-2 targeting spvC with a product of about 571 bp. One sample was not amplified by primers FSPV-1/RSPV-2 as it lacked virulence plasmid. Analysis of sequences indicated 100% homology with closely related serovars of S. enterica subspecies enterica serovar Typhimurium.

Conclusion: Salmonella Typhimurium that contained invA and spvC genes are pathogenic and virulent strains. Keywords: invasive gene A, polymerase chain reaction, Salmonella plasmid virulence gene, Salmonella Typhimurium, sequencing.

Keywords: invasive gene A, polymerase chain reaction, Salmonella plasmid virulence gene, Salmonella Typhimurium, sequencing.

How to cite this article: Mkangara M, Mbega ER, Chacha M (2020) Molecular identification of Salmonella Typhimurium from village chickens based on invA and spvC genes, Veterinary World, 13(4): 764-767.

Received: 17-09-2019 Accepted: 11-03-2020 Published online: 23-04-2020

Corresponding author: Mwanaisha Mkangara E-mail: mkangaram@yahoo.com

DOI: 10.14202/vetworld.2020.764-767

Copyright: Mkangara, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.