Vet World Vol.13 December-2020 Article-26
Research Article
Veterinary World, 13(12): 2787-2792
https://doi.org/10.14202/vetworld.2020.2787-2792
Clinical and pathological features of aspergillosis due to Aspergillus fumigatus in broilers
2. Department of Pathology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
3. Department of Bioresource and Veterinary Technology, Vocational College, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Background and Aim: Aspergillus fumigatus is a ubiquitous pathogen causing aspergillosis in poultry. This research aimed to evaluate the clinical and pathological features of aspergillosis infection in broilers.
Materials and Methods: A. fumigatus infection was induced experimentally by intra-air sac inoculation of a 1.7×108 spore suspension into broilers. Infected and non-infected birds were closely observed for the development of clinical signs of infection twice daily. Pathological samples were collected 5, 14, and 30 days post-infection (dpi) and examined by hematoxylin-eosin staining.
Results: A total of 160 birds were included in this study. Clinical signs emerged at 3 dpi and became consistent at 5 dpi. A considerable decrease in severity and number of birds showing infection symptoms followed. The clinical signs of aspergillosis included anorexia (n=40; 50%), lethargy (n=32; 40%), dyspnea (n=38; 48%), and gasping (n=29; 36%). Macroscopic changes in the air sacs at 3 dpi included the development of minor lesions showing cloudiness, slight membrane thickening, and local exudates. Histopathological examination of the air sacs collected at 3 dpi indicated local inflammation surrounded by hyphae and spores. At 5 dpi, infected birds developed nodules, necrosis, and parenchymal consolidation of the lungs. Pulmonary changes, such as bronchopneumonia, spores, septate hyphae, and mild granulomatous inflammation, were also observed. At 14 dpi, multiple caseous nodules and plaques were found in the air sacs; plaque and necrosis in large areas of the lungs and severe multifocal granulomatous inflammation were noted.
Conclusion: The clinical symptoms of aspergillosis emerged at 3 dpi and gradually decreased beginning at 7 dpi. Similar pathological changes were observed in the air sacs and lungs. The results of this work provide additional information on the pathogenesis of aspergillosis. Keywords: aspergillosis, broiler, clinical, pathological.
Keywords: aspergillosis, broiler, clinical, pathological.
How to cite this article: Nururrozi A, Yanuartono Y, Widyarini S, Ramandani D, Indarjulianto S (2020) Clinical and pathological features of aspergillosis due to Aspergillus fumigatus in broilers, Veterinary World, 13(12): 2787-2792.
Received: 14-08-2020 Accepted: 17-11-2020 Published online: 26-12-2020
Corresponding author: Soedarmanto Indarjulianto E-mail: indarjulianto@ugm.ac.id
DOI: 10.14202/vetworld.2020.2787-2792
Copyright: Nururrozi, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.