Assessment of biphasic calcium phosphate 70/30 alginate scaffold on the tibia in pigs

Vet World Vol.13 December-2020 Article-8

Research Article

Veterinary World, 13(12): 2635-2642

https://doi.org/10.14202/vetworld.2020.2635-2642

Gunanti Soeyono^1,2, Kiagus Dahlan³, Melpa Susanti Purba¹, Sus Dherthi Widhyari⁴, Rr. Soesatyoratih¹, Thang Shi Teng¹, Lieonny Budiarti¹, Ho Kin Wai¹, and Agatha Kosat¹

1. Department of Veterinary Clinic Reproduction and Pathology, Division of Veterinary Surgery and Radiology, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia.
2. Veterinary Paramedic Study Program, Vocational School, Bogor Agriculture University, Bogor, Indonesia.
3. Department of Physics, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Bogor, Indonesia.
4. Department of Veterinary Clinic Reproduction and Pathology, Division of Veterinary Medicine, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia.

Background and Aim: Calcium phosphate bioceramics have been used for at least a decade, and many investigations have focused on the use of hydroxyapatite (HA) derivative in the regeneration of bone defects. Biphasic calcium phosphate (BCP) is a biomaterial composed of HA and beta-tricalcium phosphate (BCP), with a structure similar to bone. The aim of the study was to determine the influence of the BCP/alginate scaffold on tissue growth, blood, the lungs, and the electrical activity of the heart during bone healing in the tibia of pig.

Materials and Methods: Three pigs were implanted with BCP/alginate scaffolds in the tibias. Pigs were acclimatized and treated with antibiotics and anthelminthic drugs 14 days before implantation. Each pig was implanted with a BCP/ alginate scaffold in the right tibia and a defect without the implant was made in the left tibia as the control. Radiographic images of the tibia were captured 0, 7, 30, and 60 days after the operation. Erythrograms, radiography of the lungs, and electrocardiogram (ECG) recordings were done 0, 30, and 60 days after the operation.

Results: Radiographic evaluations showed that the implant and peri-implant density of BCP decreased throughout the process of bone healing. The erythrogram profile indicated that a substantial amount of time (60 days) was required to adapt and return to pre-operative conditions. No significant differences in ECG recordings or pulmonary radiography were detected.

Conclusion: The BCP/alginate scaffold did not induce a faster recovery rate from the bone defect compared to the control with no implant. However, the BCP/alginate scaffold was biodegradable, bioresorbable, and non-toxic. Keywords: biphasic calcium phosphate, bone implant, erythrogram, pig, pulmonary radiography, scaffold.

Keywords: biphasic calcium phosphate, bone implant, erythrogram, pig, pulmonary radiography, scaffold.

How to cite this article: Soeyono G, Dahlan K, Purba MS, Widhyari SD, Rr. Soesatyoratih, Teng TS, Budiarti L, Wai HK, Kosat A (2020) Assessment of biphasic calcium phosphate 70/30 alginate scaffold on the tibia in pigs, Veterinary World, 13(12): 2635-2642.

Received: 02-07-2020 Accepted: 22-10-2020 Published online: 11-12-2020

Corresponding author: Gunanti Soeyono E-mail: gunanti.soe@gmail.com

DOI: 10.14202/vetworld.2020.2635-2642

Copyright: Soeyono, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.