Vet World Vol.13 February-2020 Article-13
Research Article
Veterinary World, 13(2): 317-325
https://doi.org/10.14202/vetworld.2020.317-325
Occurrence and antibiogram of Listeria species in raw pork, beef, and chicken meats marketed in Enugu State, Southeast Nigeria
2. Microbiology Unit, Department of Veterinary Pathology and Microbiology, University of Nigeria, 400001 Nsukka, Enugu State Nigeria.
3. Department of Veterinary Pathology, Michael Okpara University of Agriculture, 440233 Umudike, Abia State, Nigeria.
4. Department of Microbiology, University of Nigeria, 400001 Nsukka, Enugu State, Nigeria.
5. Programa de Microbiologia y Micologia, Instituto de Ciencias Biomedicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
6. Secretaria Regional Ministerial de Salud Region Metropolitana Santiago, Chile.
Background and Aim: This study was undertaken to isolate Listeria (L.) species from raw meats sold in markets in Enugu State, Southeast Nigeria, and to determine the antibacterial resistance profile.
Materials and Methods: Twenty-five grams of beef (n=144), chicken meat (n=144), and pork (n=144) were collected randomly from supermarkets and general markets in Enugu State. Isolation of Listeria was done using half and full Fraser broths, and polymyxin acriflavine lithium chloride ceftazidime aesculin mannitol agar. Identification of isolates was done using an analytical profile index kit specific for Listeria. Confirmation of the genus Listeria was done by a polymerase chain reaction. The resistance of the isolates was determined using the disk diffusion method.
Results: Listeria was isolated from 39/144 (27.1%) chicken meat, 19/144 (13.2%) pork, and 66/144 (45.8%) beef samples cultured. Listeria innocua was the predominant species in chicken meat (52.6%) and beef (81.8%) samples. Listeria grayi, Listeria welshimeri, and Listeria ivanovii were also isolated from the beef and chicken meat samples. More than 65% of the isolates were resistant to penicillin, rifampicin, ciprofloxacin, sulfamethoxazole-trimethoprim, and cephalothin. All the isolates from beef and pork samples and 23 (92%) from chicken meat samples, were resistant to ≥3 classes of antibacterial agents. Mean multiple antibiotic resistance index (MARI) was 0.77 (range=0.42-1.00), 0.58 (range=0.25-0.83), and 0.79 (range=0.58-0.92) for the isolates from beef, chicken meat, and pork samples, respectively. All the isolates had MARI >0.2.
Conclusion: Multidrug-resistant Listeria strains contaminate raw beef, pork, and chicken meats marketed in Enugu State, Southeast Nigeria. Keywords: antibacterial resistance, beef, chicken meat, Listeria species, pork.
Keywords: antibacterial resistance, beef, chicken meat, Listeria species, pork.
How to cite this article: Okorie-Kanu OJ, Anyanwu MU, Ezenduka EV, Mgbeahuruike AC, Okorie-Kanu CO, Ugwuijem EE, Idogwu MN, Anyaoha CO, Majesty-Alukagberie OL, Vidal RO, Vidal M (2020) Occurrence and antibiogram of Listeria species in raw pork, beef, and chicken meats marketed in Enugu State, Southeast Nigeria, Veterinary World, 13(2): 317-325.
Received: 11-10-2019 Accepted: 13-01-2020 Published online: 18-02-2020
Corresponding author: Madubuike Umunna Anyanwu E-mail: madubuike.anyanwu@unn.edu.ng
DOI: 10.14202/vetworld.2020.317-325
Copyright: Okorie-Kanu, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.