Immunohistochemical study to detect glucagon and insulin hormones in pancreas of camel and buffalo

Vet World Vol.13 February-2020 Article-18

Research Article

Veterinary World, 13(2): 354-359

https://doi.org/10.14202/vetworld.2020.354-359

Ali F. Bargooth¹, Jafar G. A. Ali-Jebori², Ahmed M. Al-Badri³, Ali M. R. Al-Yasari⁴, and Esraa A. Zegyer³

1. Department of Biology, College of Education for Pure Sciences, Wasit University, Iraq.
2. Department of Anatomy, College of Veterinary Medicine, Qasim Green University, Iraq.
3. Department of Biology, College of Science, Wasit University, Iraq.
4. College of Veterinary Medicine, Al-Muthanna University, Iraq.

Background and Aim: Glucagon plays a significant role in glucose homeostasis by controlling hepatic glucose output in terms of both hypoglycemic and normoglycemic conditions. This study aimed to determine the amount and intensity of insulin and glucagon in addition to estimating the relationship between α- and β-cells for two animals, camel and buffalo.

Materials and Methods: Twenty fresh pancreas samples were collected from 10 buffalo and 10 camel adults immediately after slaughter from AL-Kut abattoir, Al- Kut, Iraq. Hematoxylin and eosin staining technique and the immunohistochemistry technique were used.

Results: The histological results, for both animals, showed the cells of the pancreatic islet could be differentiated from the exocrine cells by their paler appearance. The pancreatic islets were round, oval, and irregular shaped. In the camel, the pancreatic islets had a larger diameter than that in the buffalo. The average diameter of β-cells and their percentage was higher than those of the α-cells in the camel. In the buffalo, glucagon-immunoreactive cells were found in abundance with high intensity, whereas insulin-immunoreactive cells were more prominent with high intensity in the camel. In both animals, the α-cells and glucagon-immunoreactive cells were distributed on the peripheries of the pancreatic islets, whereas the β-cells were distributed throughout the pancreatic islets.

Conclusion: The study inferences that these differences may be due to the differences in the environment of the animals which affect the structures of body organs. Keywords: buffalo, camel, glucagon, immunohistochemical, insulin, pancreas.

Keywords: buffalo, camel, glucagon, immunohistochemical, insulin, pancreas.

How to cite this article: Bargooth AF, Ali-Jebori JGA, Al-Badri AM, Al-Yasari AMR, Zegyer EA (2020) Immunohistochemical study to detect glucagon and insulin hormones in pancreas of camel and buffalo, Veterinary World, 13(2): 354-359.

Received: 13-09-2019 Accepted: 13-01-2020 Published online: 26-02-2020

Corresponding author: Ali F. Bargooth E-mail: alifayadhbargoothk1@gmail.com

DOI: 10.14202/vetworld.2020.354-359

Copyright: Bargooth, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.