Antimicrobial resistance pattern of extended-spectrum β-lactamase-producing Escherichia coli isolated from fecal samples of piglets and pig farm workers of selected organized farms of India

Vet World Vol.13 February-2020 Article-19

Research Article

Veterinary World, 13(2): 360-363

https://doi.org/10.14202/vetworld.2020.360-363

Shikha Tamta¹, Obli Rajendran Vinodh Kumar¹, Shiv Varan Singh², Bommenahalli Siddaramiah Pruthvishree¹, Ravichandran Karthikeyan¹, Ramkumar Rupner¹, Dharmendra Kumar Sinha¹, and Bhoj Raj Singh¹

1. Division of Epidemiology, ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India.
2. Division of Bacteriology and Mycology, ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India.

Background and Aim: Extended-spectrum β-lactamase (ESBL)-producing Escherichia coli are gradually increasing worldwide and carry a serious public threat. This study aimed to determine the antimicrobial resistance pattern of ESBL-producing E. coli isolated from fecal samples of piglets and pig farm workers.

Materials and Methods: Fecal samples from <3-month-old piglets (n=156) and farm workers (n=21) were processed for the isolation of ESBL-producing E. coli in MacConkey agar added with 1 μg/mL of cefotaxime. E. coli (piglets=124; farm workers=21) were tested for ESBL production by combined disk method and ESBL E-strip test. Each of the ESBL-positive isolate was subjected to antibiotic susceptibility testing. The ESBL-producing E. coli were further processed for genotypic confirmation to CTX-M gene.

Results: A total of 55 (44.4%, 55/124) and nine (42.9%, 9/21) ESBL-producing E. coli were isolated from piglets and farm workers, respectively. Antibiotic susceptibility testing of the ESBL-positive E. coli isolates from piglets and farm workers showed 100% resistance to ceftazidime, cefotaxime, cefotaxime/clavulanic acid, ceftazidime/clavulanic acid, and cefpodoxime. A proportion of 100% (55/55) and 88.9% (8/9) ESBL-positive E. coli were multidrug resistance (MDR) in piglets and farm workers, respectively. On genotypic screening of the ESBL E. coli isolated from piglets (n=55), 15 were positive for the blaCTX-M gene and of the nine ESBL E. coli from farm workers, none were positive for the blaCTX-M gene.

Conclusion: Although there was no significant difference in isolation of ESBL-producing E. coli between piglets and farm workers, the ESBL-positive E. coli from piglets showed relatively higher MDR than farm workers. Keywords: CTX-M gene, India, multidrug resistance, organized farm, piglets, workers.

Keywords: CTX-M gene, India, multidrug resistance, organized farm, piglets, workers.

How to cite this article: Tamta S, Kumar ORV, Singh SV, Pruthvishree BS, Karthikeyan R, Rupner R, Sinha DK, Singh BR (2020) Antimicrobial resistance pattern of extended-spectrum β-lactamase-producing Escherichia coli isolated from fecal samples of piglets and pig farm workers of selected organized farms of India, Veterinary World, 13(2): 360-363.

Received: 21-10-2019 Accepted: 13-01-2020 Published online: 26-02-2020

Corresponding author: Obli Rajendran Vinodh Kumar E-mail: vinodhkumar.rajendran@gmail.com

DOI: 10.14202/vetworld.2020.360-363

Copyright: Tamta, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.