Blastocystis spp. subtype 10 infected beef cattle in Kamal and Socah, Bangkalan, Madura, Indonesia

Vet World Vol.13 February-2020 Article-2

Research Article

Veterinary World, 13(2): 231-237

https://doi.org/10.14202/vetworld.2020.231-237

Lucia Tri Suwanti^1,2, Yuli Susana³, Poedji Hastutiek¹, Endang Suprihati¹, and Nunuk Dyah Retno Lastuti¹

1. Department of Veterinary Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Mulyorejo, Kampus C Unair, Surabaya, 60115, Indonesia.
2. Toxoplasmosis Study Group, Institute of Tropical Diseases, Universitas Airlangga, Jl. Mulyorejo, Campus C Unair, Surabaya 60115, Indonesia.
3. Magister Student, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Mulyorejo, Campus C Unair, Surabaya 60115, Indonesia.

Background and Aim: Blastocystis spp. is a gastrointestinal parasite that can infect both humans and animals and has the potential to become a zoonotic parasite. This study analyzed a subtype (ST) of Blastocystis spp. that had infected beef cattle in Kamal and Socah, Bangkalan, Madura, Indonesia.

Materials and Methods: Fresh stool samples were collected from 108 beef cattle at Kamal and Socah, Bangkalan, Madura, Indonesia. Blastocystis spp. were detected both morphologically and genetically based on the 18S rRNA gene. The morphology of Blastocystis spp. from the stool samples and cultured samples were observed under a light microscope. Blastocystis spp. from 20 positive cultures were amplified through polymerase chain reaction, and the resultant sequences were identified by ST.

Results: One hundred and eight (100%) fecal samples from the fresh or cultured stools were positive morphologically for Blastocystis spp. Molecularly, all 20 of the samples selected for DNA analysis were found to be Blastocystis spp. ST 10.

Conclusion: Based on morphological and molecular detection, the prevalence of Blastocystis spp. infection in beef cattle within Kamal and Socah, Bangkalan, Madura, Indonesia, was high. About 100% were non-zoonotic parasites. This was the first report of Blastocystis spp. ST 10 found in infected beef cattle in Kamal and Socah, Bangkalan, Madura, Indonesia. Keywords: beef cattle, Blastocystis spp. subtype 10, Madura, Indonesia, zoonotic.

Keywords: beef cattle, Blastocystis spp. subtype 10, Madura, Indonesia, zoonotic.

How to cite this article: Suwanti LT, Susana Y, Hastutiek P, Suprihati E, Lastuti NDR (2020) Blastocystis spp. subtype 10 infected beef cattle in Kamal and Socah, Bangkalan, Madura, Indonesia, Veterinary World, 13(2): 231-237.

Received: 08-07-2019 Accepted: 26-12-2019 Published online: 07-02-2020

Corresponding author: Lucia Tri Suwanti E-mail: tswant@gmail.com

DOI: 10.14202/vetworld.2020.231-237

Copyright: Suwanti, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.